$Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge$

Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge

The sponge (1L82008) possessed the highest antimicrobial activity among four sponges and three corals species investigated. The sponge tissue was extracted with methanol and followed with water and examined against two Gram-positive bacteria (Staphylococcus aureus and Bacillus anthracis), two gramne...

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Main Authors:	Darnis, Deny Susanti, Qaralleh, Haitham, Saad, Shahbudin, Syed Osman Idid, Syed Zahir Idid
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/16567/ http://irep.iium.edu.my/16567/ http://irep.iium.edu.my/16567/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf

id	iium-16567
recordtype	eprints
spelling	iium-165672012-01-29T12:28:10Z http://irep.iium.edu.my/16567/ Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge Darnis, Deny Susanti Qaralleh, Haitham Saad, Shahbudin Syed Osman Idid, Syed Zahir Idid Q Science (General) The sponge (1L82008) possessed the highest antimicrobial activity among four sponges and three corals species investigated. The sponge tissue was extracted with methanol and followed with water and examined against two Gram-positive bacteria (Staphylococcus aureus and Bacillus anthracis), two gramnegative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two fungi strains (Candida albicans and Cryptococcus neoformans) using disc diffusion method. The results showed that methanol extract had stronger antimicrobial activity against B. anthracis (400μg/disc) than water extract. Since the methanol extract was found to be active, it was used for further bioassay-directed fraction in order to isolate the active compounds. The dichloromethane and n-butanol fractions were found to be active at concentration 400μg/disc. Purification of n-butanol fraction was done by using normal phase column chromatography eluted with n-butanol:acetic acid:water (12:3:5). Based on thin layer chromatography (TLC) profiles, the same fractions were collected, concentrated and assayed for antimicrobial activity against B. fnthracis. The results showed that fractions (87-123) and fractions (124-183) were the most active fractions. The TLC of the active fractions were then developed using the same mobile phase and subjected to contact bioautography against B. anthracis, which showed inhibition zone at different retention time, indicating the presence of antimicrobial components. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/16567/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Darnis, Deny Susanti and Qaralleh, Haitham and Saad, Shahbudin and Syed Osman Idid, Syed Zahir Idid (2010) Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. (Unpublished) http://www.iium.edu.my/irie/10/sub10/author/list_p.php
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Darnis, Deny Susanti Qaralleh, Haitham Saad, Shahbudin Syed Osman Idid, Syed Zahir Idid Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
description	The sponge (1L82008) possessed the highest antimicrobial activity among four sponges and three corals species investigated. The sponge tissue was extracted with methanol and followed with water and examined against two Gram-positive bacteria (Staphylococcus aureus and Bacillus anthracis), two gramnegative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two fungi strains (Candida albicans and Cryptococcus neoformans) using disc diffusion method. The results showed that methanol extract had stronger antimicrobial activity against B. anthracis (400μg/disc) than water extract. Since the methanol extract was found to be active, it was used for further bioassay-directed fraction in order to isolate the active compounds. The dichloromethane and n-butanol fractions were found to be active at concentration 400μg/disc. Purification of n-butanol fraction was done by using normal phase column chromatography eluted with n-butanol:acetic acid:water (12:3:5). Based on thin layer chromatography (TLC) profiles, the same fractions were collected, concentrated and assayed for antimicrobial activity against B. fnthracis. The results showed that fractions (87-123) and fractions (124-183) were the most active fractions. The TLC of the active fractions were then developed using the same mobile phase and subjected to contact bioautography against B. anthracis, which showed inhibition zone at different retention time, indicating the presence of antimicrobial components.
format	Conference or Workshop Item
author	Darnis, Deny Susanti Qaralleh, Haitham Saad, Shahbudin Syed Osman Idid, Syed Zahir Idid
author_facet	Darnis, Deny Susanti Qaralleh, Haitham Saad, Shahbudin Syed Osman Idid, Syed Zahir Idid
author_sort	Darnis, Deny Susanti
title	Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
title_short	Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
title_full	Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
title_fullStr	Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
title_full_unstemmed	Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
title_sort	bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge
publishDate	2010
url	http://irep.iium.edu.my/16567/ http://irep.iium.edu.my/16567/ http://irep.iium.edu.my/16567/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
first_indexed	2023-09-18T20:25:21Z
last_indexed	2023-09-18T20:25:21Z
_version_	1777408379217510400

Bioassay-direct fractionation and contact bioautography technique for antimicrobial activities of marine sponge

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