Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit

The aim of the present study is to purify recombinant nucleocapsid (NP) protein of Newcastle disease virus (NDV) using microfitration fitration system and improve the processing conditions as well. A microporous membrane screening using two different sizes of membrane which are 0.1µm and 0.45µm was...

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Main Authors:	Ahmad Raus, Raha, Awang, Rus Anida, Mel, Maizirwan, Yusoff, K.
Format:	Article
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/16266/ http://irep.iium.edu.my/16266/ http://irep.iium.edu.my/16266/1/FILTRATION.pdf

id	iium-16266
recordtype	eprints
spelling	iium-162662012-01-21T03:33:16Z http://irep.iium.edu.my/16266/ Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit Ahmad Raus, Raha Awang, Rus Anida Mel, Maizirwan Yusoff, K. TP248.13 Biotechnology The aim of the present study is to purify recombinant nucleocapsid (NP) protein of Newcastle disease virus (NDV) using microfitration fitration system and improve the processing conditions as well. A microporous membrane screening using two different sizes of membrane which are 0.1µm and 0.45µm was performed before further improvement on NP protein filtration was carried out. Several conditions of the independent variables that affect the microfiltration process such as the temperature, transmembrane pressure (TMP), and viscosity were observed. It turned out to be that 0.45µm membrane gave higher yield of NP protein than that of 0.1µm membrane. Thus, 0.45µm membrane was used to improve the filtration process for NP protein. Based on the Full Factorial Design (STATISTICA 8.0, Statsoft, Inc.), eight sets of experiment were designed to identify the best conditions for the NP protein filtration. From these experiments, the optimal conditions that gave the highest yield of NP protein are; TMP 4.5psi, viscosity 2.39cP at 4ºC. Based on ANOVA (analysis of variance), all the independent variables studied do not significantly affect the purification of NP protein. However, based on the lowest p value for each variable corresponding to the response, temperature has the largest effect for the NP protein yield. IIUM Press 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/16266/1/FILTRATION.pdf Ahmad Raus, Raha and Awang, Rus Anida and Mel, Maizirwan and Yusoff, K. (2011) Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit. IIUM Engineering Journal, 12 (2). ISSN 1511-788X http://www.iium.edu.my/ejournal/index.php/iiumej/issue/view/24
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ahmad Raus, Raha Awang, Rus Anida Mel, Maizirwan Yusoff, K. Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit
description	The aim of the present study is to purify recombinant nucleocapsid (NP) protein of Newcastle disease virus (NDV) using microfitration fitration system and improve the processing conditions as well. A microporous membrane screening using two different sizes of membrane which are 0.1µm and 0.45µm was performed before further improvement on NP protein filtration was carried out. Several conditions of the independent variables that affect the microfiltration process such as the temperature, transmembrane pressure (TMP), and viscosity were observed. It turned out to be that 0.45µm membrane gave higher yield of NP protein than that of 0.1µm membrane. Thus, 0.45µm membrane was used to improve the filtration process for NP protein. Based on the Full Factorial Design (STATISTICA 8.0, Statsoft, Inc.), eight sets of experiment were designed to identify the best conditions for the NP protein filtration. From these experiments, the optimal conditions that gave the highest yield of NP protein are; TMP 4.5psi, viscosity 2.39cP at 4ºC. Based on ANOVA (analysis of variance), all the independent variables studied do not significantly affect the purification of NP protein. However, based on the lowest p value for each variable corresponding to the response, temperature has the largest effect for the NP protein yield.
format	Article
author	Ahmad Raus, Raha Awang, Rus Anida Mel, Maizirwan Yusoff, K.
author_facet	Ahmad Raus, Raha Awang, Rus Anida Mel, Maizirwan Yusoff, K.
author_sort	Ahmad Raus, Raha
title	Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit
title_short	Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit
title_full	Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit
title_fullStr	Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit
title_full_unstemmed	Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit
title_sort	purification of nucleocapsid protein of newcastle disease virus for immunodiagnostic kit
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/16266/ http://irep.iium.edu.my/16266/ http://irep.iium.edu.my/16266/1/FILTRATION.pdf
first_indexed	2023-09-18T20:25:08Z
last_indexed	2023-09-18T20:25:08Z
_version_	1777408366173224960

Purification of nucleocapsid protein of Newcastle Disease virus for immunodiagnostic kit

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