An isolated bacterial consortium for crude oil biodegradation

Crude oil utilizing bacteria were isolated from crude-oil contaminated soil (COCS) and tested for their ability to degrade COCS. Fifty strains were isolated, purified and monitored on agar plated for their growth. Twenty strains were selected for the second monitoring in shake flask using minimal...

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Main Authors:	Al-Khatib, Ma An Fahmi Rashid, Alam, Md. Zahangir, Muyibi, Suleyman Aremu, Husain, Iman A. F.
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	TP Chemical technology
Online Access:	http://irep.iium.edu.my/16233/ http://irep.iium.edu.my/16233/ http://irep.iium.edu.my/16233/ http://irep.iium.edu.my/16233/4/Alkhatib_et_al_16233.pdf

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spelling	iium-162332017-08-10T02:38:43Z http://irep.iium.edu.my/16233/ An isolated bacterial consortium for crude oil biodegradation Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Husain, Iman A. F. TP Chemical technology Crude oil utilizing bacteria were isolated from crude-oil contaminated soil (COCS) and tested for their ability to degrade COCS. Fifty strains were isolated, purified and monitored on agar plated for their growth. Twenty strains were selected for the second monitoring in shake flask using minimal salt medium with crude oil as sole carbon source. The growth was monitored by measuring the optical density (OD600) on daily basis. The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined in accordance with their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. The first order kinetic study showed that the µmax of Cons.1 was 0.02 h -1 while the half saturation constant ks was 2.2 %v/v TPH. The strains forming IMNC201 belong to Klebsiella pneumoniae and Enterobacteriaceae species. The bioremediation experiments were designed using DesignExpert 6.0.8 software by optimizing the amount of crude oil, microbial inoculum and sludge which are initially added to the autoclaved soil. Fifteen runs were carried out until no more microbial activity was noticed. The bioremediation results were compared with natural attenuation and biostimulation to determine the feasibility of the bioremediation process. From the optimization of the bioremediation, the optimum degradation obtained was 98.8% within 4 days at 5% v/w crude oil, 5% v/w inoculum and 10% v/w sludge. Biostimulation showed 98.3% TPH removal, while natural attenuation resulted in 97.7% in 13 days. The removal of crude oil by bioremediation was achieved in less time as compared to natural attenuation and biostimulation. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/16233/4/Alkhatib_et_al_16233.pdf Al-Khatib, Ma An Fahmi Rashid and Alam, Md. Zahangir and Muyibi, Suleyman Aremu and Husain, Iman A. F. (2011) An isolated bacterial consortium for crude oil biodegradation. African Journal of Biotechnology, 10 (81). pp. 18763-18767. ISSN 1684–5315 http://www.academicjournals.org/ajb/abstracts/abs2011/16DecConf/Alkhatib%20et%20al.htm DOI: 10.5897/AJB11.274
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Husain, Iman A. F. An isolated bacterial consortium for crude oil biodegradation
description	Crude oil utilizing bacteria were isolated from crude-oil contaminated soil (COCS) and tested for their ability to degrade COCS. Fifty strains were isolated, purified and monitored on agar plated for their growth. Twenty strains were selected for the second monitoring in shake flask using minimal salt medium with crude oil as sole carbon source. The growth was monitored by measuring the optical density (OD600) on daily basis. The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined in accordance with their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. The first order kinetic study showed that the µmax of Cons.1 was 0.02 h -1 while the half saturation constant ks was 2.2 %v/v TPH. The strains forming IMNC201 belong to Klebsiella pneumoniae and Enterobacteriaceae species. The bioremediation experiments were designed using DesignExpert 6.0.8 software by optimizing the amount of crude oil, microbial inoculum and sludge which are initially added to the autoclaved soil. Fifteen runs were carried out until no more microbial activity was noticed. The bioremediation results were compared with natural attenuation and biostimulation to determine the feasibility of the bioremediation process. From the optimization of the bioremediation, the optimum degradation obtained was 98.8% within 4 days at 5% v/w crude oil, 5% v/w inoculum and 10% v/w sludge. Biostimulation showed 98.3% TPH removal, while natural attenuation resulted in 97.7% in 13 days. The removal of crude oil by bioremediation was achieved in less time as compared to natural attenuation and biostimulation.
format	Article
author	Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Husain, Iman A. F.
author_facet	Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Husain, Iman A. F.
author_sort	Al-Khatib, Ma An Fahmi Rashid
title	An isolated bacterial consortium for crude oil biodegradation
title_short	An isolated bacterial consortium for crude oil biodegradation
title_full	An isolated bacterial consortium for crude oil biodegradation
title_fullStr	An isolated bacterial consortium for crude oil biodegradation
title_full_unstemmed	An isolated bacterial consortium for crude oil biodegradation
title_sort	isolated bacterial consortium for crude oil biodegradation
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/16233/ http://irep.iium.edu.my/16233/ http://irep.iium.edu.my/16233/ http://irep.iium.edu.my/16233/4/Alkhatib_et_al_16233.pdf
first_indexed	2023-09-18T20:25:07Z
last_indexed	2023-09-18T20:25:07Z
_version_	1777408364758695936

An isolated bacterial consortium for crude oil biodegradation

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