Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis

Cellulose is the main component in plant cell wall and thus the most abundant biopolymer on earth. Endoglucanase is one of the key constituent of a complex multi-enzyme system collectively known as cellulase which degrades cellulose into glucose. Cellulase has been used in various industries such as...

Full description

Bibliographic Details
Main Authors:	Moukit, Farid, Amid, Azura, Mohd. Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/16202/ http://irep.iium.edu.my/16202/ http://irep.iium.edu.my/16202/1/Expression_of_a_Fusarium_oxysporum_Endoglucanase_in_Kluyveromyces_lactis.pdf

id	iium-16202
recordtype	eprints
spelling	iium-162022012-02-02T07:18:01Z http://irep.iium.edu.my/16202/ Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis Moukit, Farid Amid, Azura Mohd. Salleh, Hamzah TP248.13 Biotechnology Cellulose is the main component in plant cell wall and thus the most abundant biopolymer on earth. Endoglucanase is one of the key constituent of a complex multi-enzyme system collectively known as cellulase which degrades cellulose into glucose. Cellulase has been used in various industries such as textile, detergent, food and feed, pulp and paper, and recently in bio-fuel industries. This research intends to clone and characterize functional endoglucanase enzyme from a thermophilic pathogenic plant fungus, Fusarium oxysporum, in suitable eukaryotic expression system (Kluyveromyces lactis). Kluyveromyces lactis have many advantages of higher eukaryotic expression systems such as: protein processing, protein folding, and post-translational modification. While being as easy to manipulate as ‘E. coli’, they give higher expression levels. This research will also optimize the process conditions required for the maximal formation of functional recombinant endoglucanase, followed by purification of the recombinant enzyme before determining the characteristics of the recombinant endoglucanase such as stability (pH, temperature) and activity (substrates, effectors and inhibitors). 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/16202/1/Expression_of_a_Fusarium_oxysporum_Endoglucanase_in_Kluyveromyces_lactis.pdf Moukit, Farid and Amid, Azura and Mohd. Salleh, Hamzah (2011) Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Moukit, Farid Amid, Azura Mohd. Salleh, Hamzah Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
description	Cellulose is the main component in plant cell wall and thus the most abundant biopolymer on earth. Endoglucanase is one of the key constituent of a complex multi-enzyme system collectively known as cellulase which degrades cellulose into glucose. Cellulase has been used in various industries such as textile, detergent, food and feed, pulp and paper, and recently in bio-fuel industries. This research intends to clone and characterize functional endoglucanase enzyme from a thermophilic pathogenic plant fungus, Fusarium oxysporum, in suitable eukaryotic expression system (Kluyveromyces lactis). Kluyveromyces lactis have many advantages of higher eukaryotic expression systems such as: protein processing, protein folding, and post-translational modification. While being as easy to manipulate as ‘E. coli’, they give higher expression levels. This research will also optimize the process conditions required for the maximal formation of functional recombinant endoglucanase, followed by purification of the recombinant enzyme before determining the characteristics of the recombinant endoglucanase such as stability (pH, temperature) and activity (substrates, effectors and inhibitors).
format	Conference or Workshop Item
author	Moukit, Farid Amid, Azura Mohd. Salleh, Hamzah
author_facet	Moukit, Farid Amid, Azura Mohd. Salleh, Hamzah
author_sort	Moukit, Farid
title	Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
title_short	Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
title_full	Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
title_fullStr	Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
title_full_unstemmed	Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
title_sort	expression of a fusarium oxysporum endoglucanase in kluyveromyces lactis
publishDate	2011
url	http://irep.iium.edu.my/16202/ http://irep.iium.edu.my/16202/ http://irep.iium.edu.my/16202/1/Expression_of_a_Fusarium_oxysporum_Endoglucanase_in_Kluyveromyces_lactis.pdf
first_indexed	2023-09-18T20:25:05Z
last_indexed	2023-09-18T20:25:05Z
_version_	1777408362529423360

Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis

Similar Items