Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)

Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the �- position of 3-carbon alkanoic acids. Pseud...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Hamid, A. A. A, Zulkifly, Nurul Asma Hasliza, Hamdan, Sallehuddin, Ariffin, Shahrul Hisham Zainal, Huyop, Fahrul Zaman
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/1606/ http://irep.iium.edu.my/1606/ http://irep.iium.edu.my/1606/1/PaperFahrul_TgAbdul_Hamid.pdf

id	iium-1606
recordtype	eprints
spelling	iium-16062011-09-05T06:43:10Z http://irep.iium.edu.my/1606/ Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP) Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman Q Science (General) Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the �- position of 3-carbon alkanoic acids. Pseudomonas sp. B6P was originally isolated from paddy field which was able to degrade 3CP therefore, suggesting it may have some desirable properties. The enzyme was purified from cell-free extracts having a monomer of 56,000 Da. It was found to be stable between pH 5 to 8 and its optimal pH was 8. Its activity was not affected by metal ions such as Mn2+, Fe3+ and Mg2+, but was inhibited by Hg2+ and Ag2+. The enzyme is specific for 3CP, and the Km value (0.20 mM ± 0.05). Academic Journals 2011-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/1606/1/PaperFahrul_TgAbdul_Hamid.pdf Tengku Abdul Hamid, Tengku Haziyamin and Hamid, A. A. A and Zulkifly, Nurul Asma Hasliza and Hamdan, Sallehuddin and Ariffin, Shahrul Hisham Zainal and Huyop, Fahrul Zaman (2011) Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP). African Journal of Biotechnology, 10 (4). pp. 610-614. ISSN ISSN 1684–5315 http://www.academicjournals.org/AJB
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
description	Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the �- position of 3-carbon alkanoic acids. Pseudomonas sp. B6P was originally isolated from paddy field which was able to degrade 3CP therefore, suggesting it may have some desirable properties. The enzyme was purified from cell-free extracts having a monomer of 56,000 Da. It was found to be stable between pH 5 to 8 and its optimal pH was 8. Its activity was not affected by metal ions such as Mn2+, Fe3+ and Mg2+, but was inhibited by Hg2+ and Ag2+. The enzyme is specific for 3CP, and the Km value (0.20 mM ± 0.05).
format	Article
author	Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Hamid, A. A. A Zulkifly, Nurul Asma Hasliza Hamdan, Sallehuddin Ariffin, Shahrul Hisham Zainal Huyop, Fahrul Zaman
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
title_short	Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
title_full	Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
title_fullStr	Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
title_full_unstemmed	Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)
title_sort	purification and properties of a new dehalogenase enzyme from pseudomonas sp. b6p grow in 3- chloropropionate (3cp)
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/1606/ http://irep.iium.edu.my/1606/ http://irep.iium.edu.my/1606/1/PaperFahrul_TgAbdul_Hamid.pdf
first_indexed	2023-09-18T20:09:00Z
last_indexed	2023-09-18T20:09:00Z
_version_	1777407350976544768

Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3- chloropropionate (3CP)

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