Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic

Lactic acid bacteria (LAB) are important inhabitants of animal intestine and are useful source of probiotic microorganism. Non-roiler chicken could be an ideal source of probiotic that can be utilized in large scale feeding of broiler chicken. In this study, fifty (50) bacterial strains that were ra...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Ezani, Ezureen, Khan Chowdhury, Ahmed Jalal
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/15911/ http://irep.iium.edu.my/15911/ http://irep.iium.edu.my/15911/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf

id	iium-15911
recordtype	eprints
spelling	iium-159112017-12-06T04:31:40Z http://irep.iium.edu.my/15911/ Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic Tengku Abdul Hamid, Tengku Haziyamin Ezani, Ezureen Khan Chowdhury, Ahmed Jalal TP248.13 Biotechnology Lactic acid bacteria (LAB) are important inhabitants of animal intestine and are useful source of probiotic microorganism. Non-roiler chicken could be an ideal source of probiotic that can be utilized in large scale feeding of broiler chicken. In this study, fifty (50) bacterial strains that were randomly isolated from the gastrointestinal tract of non-broiler chicken (Gallus gallus) prepared in 5 ml of Phosphate Buffered Saline (PBS) were tested for the identification of LAB. LAB was identified by morphological and biochemical tests. Out of the fifty (50) isolates, eleven (11) isolates were Gram-positive cocci. The eleven (11) isolates have the ability to utilize lactose as part of their metabolism process and all showed negative reactions towards catalase test. The identified strains were then screened for their inhibitory effects against pathogenic bacteria by the disc diffusion method. Eleven (11) strains of LAB isolated produced antimicrobial compounds that were active against pathogenic bacteria especially Salmonella typhimurium strains. The disc dipped in broth containing LAB inhibited the growth of pathogenic bacteria by creating an inhibition zone around the disc. With all these tests, LAB were successfully isolated and screened from gastrointestinal tract of non-broiler chicken for potential probiotic purposes. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/15911/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Tengku Abdul Hamid, Tengku Haziyamin and Ezani, Ezureen and Khan Chowdhury, Ahmed Jalal (2010) Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Tengku Abdul Hamid, Tengku Haziyamin Ezani, Ezureen Khan Chowdhury, Ahmed Jalal Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic
description	Lactic acid bacteria (LAB) are important inhabitants of animal intestine and are useful source of probiotic microorganism. Non-roiler chicken could be an ideal source of probiotic that can be utilized in large scale feeding of broiler chicken. In this study, fifty (50) bacterial strains that were randomly isolated from the gastrointestinal tract of non-broiler chicken (Gallus gallus) prepared in 5 ml of Phosphate Buffered Saline (PBS) were tested for the identification of LAB. LAB was identified by morphological and biochemical tests. Out of the fifty (50) isolates, eleven (11) isolates were Gram-positive cocci. The eleven (11) isolates have the ability to utilize lactose as part of their metabolism process and all showed negative reactions towards catalase test. The identified strains were then screened for their inhibitory effects against pathogenic bacteria by the disc diffusion method. Eleven (11) strains of LAB isolated produced antimicrobial compounds that were active against pathogenic bacteria especially Salmonella typhimurium strains. The disc dipped in broth containing LAB inhibited the growth of pathogenic bacteria by creating an inhibition zone around the disc. With all these tests, LAB were successfully isolated and screened from gastrointestinal tract of non-broiler chicken for potential probiotic purposes.
format	Conference or Workshop Item
author	Tengku Abdul Hamid, Tengku Haziyamin Ezani, Ezureen Khan Chowdhury, Ahmed Jalal
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Ezani, Ezureen Khan Chowdhury, Ahmed Jalal
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic
title_short	Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic
title_full	Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic
title_fullStr	Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic
title_full_unstemmed	Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic
title_sort	isolation and screening of lactic acid bacteria (lab) from non-broiler chicken (gallus gallus) for potential probiotic
publishDate	2010
url	http://irep.iium.edu.my/15911/ http://irep.iium.edu.my/15911/ http://irep.iium.edu.my/15911/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
first_indexed	2023-09-18T20:24:49Z
last_indexed	2023-09-18T20:24:49Z
_version_	1777408346092994560

Isolation and screening of lactic acid bacteria (LAB) from non-broiler chicken (Gallus gallus) for potential probiotic

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