Regeneration of Jatropha curcas using leaf explants

A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained...

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Main Authors: Pappusamy, Arokiaraj, S.A, Lydia, L., Norshahidah, K, Latiffah
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/15906/
http://irep.iium.edu.my/15906/
http://irep.iium.edu.my/15906/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
id iium-15906
recordtype eprints
spelling iium-159062012-01-30T01:46:17Z http://irep.iium.edu.my/15906/ Regeneration of Jatropha curcas using leaf explants Pappusamy, Arokiaraj S.A, Lydia L., Norshahidah K, Latiffah TP248.13 Biotechnology A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained in light and dark on MS medium supplemented with 0.5 mg l-1 BA and 0.5 mg l-1 IBA. The best response for shoot induction and shoot multiplication was on treatments containing (1.0 – 2.0 mg l-1) BA. The shoots were elongated and rooted on ½ strength MS medium containing 1.0 mg l-1 NAA (Napthalene Acetic Acid). 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/15906/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Pappusamy, Arokiaraj and S.A, Lydia and L., Norshahidah and K, Latiffah (2010) Regeneration of Jatropha curcas using leaf explants. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Pappusamy, Arokiaraj
S.A, Lydia
L., Norshahidah
K, Latiffah
Regeneration of Jatropha curcas using leaf explants
description A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained in light and dark on MS medium supplemented with 0.5 mg l-1 BA and 0.5 mg l-1 IBA. The best response for shoot induction and shoot multiplication was on treatments containing (1.0 – 2.0 mg l-1) BA. The shoots were elongated and rooted on ½ strength MS medium containing 1.0 mg l-1 NAA (Napthalene Acetic Acid).
format Conference or Workshop Item
author Pappusamy, Arokiaraj
S.A, Lydia
L., Norshahidah
K, Latiffah
author_facet Pappusamy, Arokiaraj
S.A, Lydia
L., Norshahidah
K, Latiffah
author_sort Pappusamy, Arokiaraj
title Regeneration of Jatropha curcas using leaf explants
title_short Regeneration of Jatropha curcas using leaf explants
title_full Regeneration of Jatropha curcas using leaf explants
title_fullStr Regeneration of Jatropha curcas using leaf explants
title_full_unstemmed Regeneration of Jatropha curcas using leaf explants
title_sort regeneration of jatropha curcas using leaf explants
publishDate 2010
url http://irep.iium.edu.my/15906/
http://irep.iium.edu.my/15906/
http://irep.iium.edu.my/15906/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
first_indexed 2023-09-18T20:24:49Z
last_indexed 2023-09-18T20:24:49Z
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