Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter

Pharmaceuticals produced in the latex cytosol of genetically transformed Hevea can be harvested nondestructively by conventional tapping. This study evaluates the promoter activity of the 5’-untranslated upstream regulating region of hevein gene, which encodes the most abundant soluble protein in H...

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Main Authors:	Pappusamy, Arokiaraj, Shuib, Siti Shuhada, Badaruddin, Badrul Ezam, Sunderasan, E.
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf

id	iium-15900
recordtype	eprints
spelling	iium-159002012-01-30T00:06:48Z http://irep.iium.edu.my/15900/ Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E. TP248.13 Biotechnology Pharmaceuticals produced in the latex cytosol of genetically transformed Hevea can be harvested nondestructively by conventional tapping. This study evaluates the promoter activity of the 5’-untranslated upstream regulating region of hevein gene, which encodes the most abundant soluble protein in Hevea latex. Constructs were prepared with the test hevein promoter fragments Hev P1 (0.35kb), Hev P2 (0.45kb) and Hev P3 (0.73kb) that were inserted 5’ to the pharmaceutical genes i.e. human protamine 1 (HP1) and human atrial natriur tic factor (HANF), in pGPTV-Kan expression vector. The expression vectors containing HP1 and HANF were electroporated into Agrobacterium tumefaciens GV2260 containing supervirulent plasmid pToK47, which were then used to infect Hevea anther callus. The growth of the putative transformed Hevea callus was monitored on kanamycin selection media. The presence of the pharmaceutical genes (HP1 and HANF), the hevein promoter fragments, and nptII selection marker were verified by PCR on sampled putative transformed callus. The remaining callus tissues will be sub-cultured and transferred into differentiation media for embryoids formation and plantlet regeneration. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/15900/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Pappusamy, Arokiaraj and Shuib, Siti Shuhada and Badaruddin, Badrul Ezam and Sunderasan, E. (2010) Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E. Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
description	Pharmaceuticals produced in the latex cytosol of genetically transformed Hevea can be harvested nondestructively by conventional tapping. This study evaluates the promoter activity of the 5’-untranslated upstream regulating region of hevein gene, which encodes the most abundant soluble protein in Hevea latex. Constructs were prepared with the test hevein promoter fragments Hev P1 (0.35kb), Hev P2 (0.45kb) and Hev P3 (0.73kb) that were inserted 5’ to the pharmaceutical genes i.e. human protamine 1 (HP1) and human atrial natriur tic factor (HANF), in pGPTV-Kan expression vector. The expression vectors containing HP1 and HANF were electroporated into Agrobacterium tumefaciens GV2260 containing supervirulent plasmid pToK47, which were then used to infect Hevea anther callus. The growth of the putative transformed Hevea callus was monitored on kanamycin selection media. The presence of the pharmaceutical genes (HP1 and HANF), the hevein promoter fragments, and nptII selection marker were verified by PCR on sampled putative transformed callus. The remaining callus tissues will be sub-cultured and transferred into differentiation media for embryoids formation and plantlet regeneration.
format	Conference or Workshop Item
author	Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E.
author_facet	Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E.
author_sort	Pappusamy, Arokiaraj
title	Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
title_short	Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
title_full	Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
title_fullStr	Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
title_full_unstemmed	Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
title_sort	hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
publishDate	2010
url	http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
first_indexed	2023-09-18T20:24:48Z
last_indexed	2023-09-18T20:24:48Z
_version_	1777408345367379968

Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter

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