Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)

Patchouli has huge potential to serve the pharmaceutical industries due to its fragrance and aromatic essential oils. To serve the needs of the pharmaceutical and perfume industries, there is an urgent need to mass propagate clonal copies of the desired patchouli plantlets. In order to generate gene...

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Main Authors:	Pappusamy, Arokiaraj, Fitriana, Sita, Salihon, Jailani, Amalina, Ida
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/15872/ http://irep.iium.edu.my/15872/ http://irep.iium.edu.my/15872/1/direct_regereneration_and_characterization_of_plant.pdf

id	iium-15872
recordtype	eprints
spelling	iium-158722012-01-29T23:30:25Z http://irep.iium.edu.my/15872/ Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth) Pappusamy, Arokiaraj Fitriana, Sita Salihon, Jailani Amalina, Ida Q Science (General) Patchouli has huge potential to serve the pharmaceutical industries due to its fragrance and aromatic essential oils. To serve the needs of the pharmaceutical and perfume industries, there is an urgent need to mass propagate clonal copies of the desired patchouli plantlets. In order to generate genetically identical clonal copies of the desired cultivar, direct regeneration of patchouli plantlets by tissue culture using leaf explants was successful. The tissue culture formulation for direct regeneration employed Murashige and Skoog medium with 0,5 mg/l 2,4 D (2,4Dichlorofenoksiacetat) in combination with (0, 0.25, 0.5, 0.75, 1 mg/l) BAP (Benzyl Aminopurine). The best response for direct shoot regeneration was observed after 35 days occurred in media containing 0.75 mg/l of BAP without 2,4 D and this gave the highest efficiency of regeneration frequency of leaf (88%) and highest number of shoot per explant (102). For elongation of shoots, MS basal medium gave a better response compared to ½ MS Medium. The average shoot elongation was about 3.3 cm and this gave an average of 7 leaflets per shoot. Elongated shoots were rooted and leaves growth using MS basal liquid medium and MS basal solid medium with or without 1 mg/l NAA (1-Naphthalene acetic acid). The best response for root induction and leaves growth was observed using MS basal liquid medium without NAA and this gave a frequency of 12 roots per shoot with an average length of 2.75 cm. The regenerated plantlets were acclimatized and hardened by transferring the test-tube plantlets into sterile water for 2 days. The plantlets were successfully established in soil containing compost and top soil (1:1) and the frequency of establishment was 90%. The clonal plantlets were verified using RAPD analysis. The results showed a very high frequency of true-to-type plantlets. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/15872/1/direct_regereneration_and_characterization_of_plant.pdf Pappusamy, Arokiaraj and Fitriana, Sita and Salihon, Jailani and Amalina, Ida (2010) Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth). In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Pappusamy, Arokiaraj Fitriana, Sita Salihon, Jailani Amalina, Ida Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)
description	Patchouli has huge potential to serve the pharmaceutical industries due to its fragrance and aromatic essential oils. To serve the needs of the pharmaceutical and perfume industries, there is an urgent need to mass propagate clonal copies of the desired patchouli plantlets. In order to generate genetically identical clonal copies of the desired cultivar, direct regeneration of patchouli plantlets by tissue culture using leaf explants was successful. The tissue culture formulation for direct regeneration employed Murashige and Skoog medium with 0,5 mg/l 2,4 D (2,4Dichlorofenoksiacetat) in combination with (0, 0.25, 0.5, 0.75, 1 mg/l) BAP (Benzyl Aminopurine). The best response for direct shoot regeneration was observed after 35 days occurred in media containing 0.75 mg/l of BAP without 2,4 D and this gave the highest efficiency of regeneration frequency of leaf (88%) and highest number of shoot per explant (102). For elongation of shoots, MS basal medium gave a better response compared to ½ MS Medium. The average shoot elongation was about 3.3 cm and this gave an average of 7 leaflets per shoot. Elongated shoots were rooted and leaves growth using MS basal liquid medium and MS basal solid medium with or without 1 mg/l NAA (1-Naphthalene acetic acid). The best response for root induction and leaves growth was observed using MS basal liquid medium without NAA and this gave a frequency of 12 roots per shoot with an average length of 2.75 cm. The regenerated plantlets were acclimatized and hardened by transferring the test-tube plantlets into sterile water for 2 days. The plantlets were successfully established in soil containing compost and top soil (1:1) and the frequency of establishment was 90%. The clonal plantlets were verified using RAPD analysis. The results showed a very high frequency of true-to-type plantlets.
format	Conference or Workshop Item
author	Pappusamy, Arokiaraj Fitriana, Sita Salihon, Jailani Amalina, Ida
author_facet	Pappusamy, Arokiaraj Fitriana, Sita Salihon, Jailani Amalina, Ida
author_sort	Pappusamy, Arokiaraj
title	Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)
title_short	Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)
title_full	Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)
title_fullStr	Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)
title_full_unstemmed	Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)
title_sort	direct regeneration and characterization of plant derived from leaf in vitro culture patchouli ( pogostemon cablin, benth)
publishDate	2010
url	http://irep.iium.edu.my/15872/ http://irep.iium.edu.my/15872/ http://irep.iium.edu.my/15872/1/direct_regereneration_and_characterization_of_plant.pdf
first_indexed	2023-09-18T20:24:46Z
last_indexed	2023-09-18T20:24:46Z
_version_	1777408343445340160

Direct regeneration and characterization of plant derived from leaf in vitro culture Patchouli ( Pogostemon cablin, Benth)

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