Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f

Objective: The paper reported chemical constituents of stembark of Garcinia malaccensis together with apoptotic, antimicrobial and antioxidant activities. Methods: Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan bl...

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Main Authors:	Bakhtiar, M. Taher, Darnis, Deny Susanti, Rezali, Mohd Fazlin, Ahmad Zohri, Farah Syahidah, Solachuddin Jauhari, Arief Ichwan, Alkhamaiseh, Suhaib Ibrahim
Format:	Article
Language:	English
Published:	Elsevier 2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/14544/ http://irep.iium.edu.my/14544/ http://irep.iium.edu.my/14544/1/Asian_Pac_J_Trop_Med.pdf

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recordtype	eprints
spelling	iium-145442014-12-09T00:24:51Z http://irep.iium.edu.my/14544/ Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f Bakhtiar, M. Taher Darnis, Deny Susanti Rezali, Mohd Fazlin Ahmad Zohri, Farah Syahidah Solachuddin Jauhari, Arief Ichwan Alkhamaiseh, Suhaib Ibrahim RS Pharmacy and materia medica Objective: The paper reported chemical constituents of stembark of Garcinia malaccensis together with apoptotic, antimicrobial and antioxidant activities. Methods: Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Antibacterial activity was conducted by disc diffusion and microdilution methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging. Results: The phytochemical study led to the isolation of -mangostin and cycloart-24-en-3-ol. -Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC50 of 0.33 M. β- and α-mangostin showed activity against K562 cells with IC50 of 0.40 M and 0.48 M, respectively. -Mangostin was active against Gram-positive bacteria, Staphylococcus aureus and Bacilus anthracis with inhibition zone and MIC value of (19 mm; 0.025 mg/mL) and (20 mm; 0.013 mg/mL), respectively. In antioxidant assay, -mangostin exhibited activity as an inhibitor of lipid peroxidation. Conclusion: G. malaccensis presence - and -mangostin and cycloart-24-en-3-ol. -Mangostin was found very active against HSC-3 cells and K562. The results suggest that mangostins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis. In addition, -and -mangostin was found inhibit the growth of Gram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation. Elsevier 2012-02-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14544/1/Asian_Pac_J_Trop_Med.pdf Bakhtiar, M. Taher and Darnis, Deny Susanti and Rezali, Mohd Fazlin and Ahmad Zohri, Farah Syahidah and Solachuddin Jauhari, Arief Ichwan and Alkhamaiseh, Suhaib Ibrahim (2012) Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f. Asian Pacific Journal of Tropical Medicine, 5 (2). pp. 136-141. ISSN 1995-7645 http://www.sciencedirect.com/science/journal/19957645
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Darnis, Deny Susanti Rezali, Mohd Fazlin Ahmad Zohri, Farah Syahidah Solachuddin Jauhari, Arief Ichwan Alkhamaiseh, Suhaib Ibrahim Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f
description	Objective: The paper reported chemical constituents of stembark of Garcinia malaccensis together with apoptotic, antimicrobial and antioxidant activities. Methods: Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Antibacterial activity was conducted by disc diffusion and microdilution methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging. Results: The phytochemical study led to the isolation of -mangostin and cycloart-24-en-3-ol. -Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC50 of 0.33 M. β- and α-mangostin showed activity against K562 cells with IC50 of 0.40 M and 0.48 M, respectively. -Mangostin was active against Gram-positive bacteria, Staphylococcus aureus and Bacilus anthracis with inhibition zone and MIC value of (19 mm; 0.025 mg/mL) and (20 mm; 0.013 mg/mL), respectively. In antioxidant assay, -mangostin exhibited activity as an inhibitor of lipid peroxidation. Conclusion: G. malaccensis presence - and -mangostin and cycloart-24-en-3-ol. -Mangostin was found very active against HSC-3 cells and K562. The results suggest that mangostins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis. In addition, -and -mangostin was found inhibit the growth of Gram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation.
format	Article
author	Bakhtiar, M. Taher Darnis, Deny Susanti Rezali, Mohd Fazlin Ahmad Zohri, Farah Syahidah Solachuddin Jauhari, Arief Ichwan Alkhamaiseh, Suhaib Ibrahim
author_facet	Bakhtiar, M. Taher Darnis, Deny Susanti Rezali, Mohd Fazlin Ahmad Zohri, Farah Syahidah Solachuddin Jauhari, Arief Ichwan Alkhamaiseh, Suhaib Ibrahim
author_sort	Bakhtiar, M. Taher
title	Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f
title_short	Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f
title_full	Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f
title_fullStr	Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f
title_full_unstemmed	Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f
title_sort	apoptosis, antimicrobial and antioxidant activities of phytochemicals from garcinia malaccensis hk.f
publisher	Elsevier
publishDate	2012
url	http://irep.iium.edu.my/14544/ http://irep.iium.edu.my/14544/ http://irep.iium.edu.my/14544/1/Asian_Pac_J_Trop_Med.pdf
first_indexed	2023-09-18T20:23:43Z
last_indexed	2023-09-18T20:23:43Z
_version_	1777408277341011968

Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f

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