Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification

To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill...

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Main Authors:	Rashid, Shah S, Alam, Md Zahangir, Abdulkarim, Mohd Ismail, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/14380/ http://irep.iium.edu.my/14380/ http://irep.iium.edu.my/14380/ http://irep.iium.edu.my/14380/1/AJB-Rashid_et_al.pdf

id	iium-14380
recordtype	eprints
spelling	iium-143802017-08-10T02:32:48Z http://irep.iium.edu.my/14380/ Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification Rashid, Shah S, Alam, Md Zahangir Abdulkarim, Mohd Ismail Mohd. Salleh, Hamzah TP248.13 Biotechnology To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain, Trichoderma reesei RUT C-30. This study was conducted by using two different types of agents (physical and chemical). Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for the pretreatment of EFB to enhance the enzymatic saccharification of EFB. NaOH was proved to be the best among all the pretreatment agents and 3% NaOH was far higher and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/g of EFB) after 96 h of saccharification. A maximum of 41.82% yield of reducing sugar was achieved with 5% (w/v) of EFB and 7% (v/v) of enzyme after 120 h of saccharification when eight important parameters, namely saccharification duration, EFB size, EFB dose, enzyme dose, Tween 80, triton 100, agitation and incubation temperature, were examined in an OFAT (one factor at-a-time) design. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14380/1/AJB-Rashid_et_al.pdf Rashid, Shah S, and Alam, Md Zahangir and Abdulkarim, Mohd Ismail and Mohd. Salleh, Hamzah (2011) Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification. African Journal of Biotechnology, 10 (81). pp. 18728-18738. ISSN 1684–5315 http://dx.doi.org/10.5897/AJB11.2745 doi:10.5897/AJB11.2745
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Rashid, Shah S, Alam, Md Zahangir Abdulkarim, Mohd Ismail Mohd. Salleh, Hamzah Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
description	To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain, Trichoderma reesei RUT C-30. This study was conducted by using two different types of agents (physical and chemical). Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for the pretreatment of EFB to enhance the enzymatic saccharification of EFB. NaOH was proved to be the best among all the pretreatment agents and 3% NaOH was far higher and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/g of EFB) after 96 h of saccharification. A maximum of 41.82% yield of reducing sugar was achieved with 5% (w/v) of EFB and 7% (v/v) of enzyme after 120 h of saccharification when eight important parameters, namely saccharification duration, EFB size, EFB dose, enzyme dose, Tween 80, triton 100, agitation and incubation temperature, were examined in an OFAT (one factor at-a-time) design.
format	Article
author	Rashid, Shah S, Alam, Md Zahangir Abdulkarim, Mohd Ismail Mohd. Salleh, Hamzah
author_facet	Rashid, Shah S, Alam, Md Zahangir Abdulkarim, Mohd Ismail Mohd. Salleh, Hamzah
author_sort	Rashid, Shah S,
title	Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_short	Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_full	Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_fullStr	Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_full_unstemmed	Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
title_sort	development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/14380/ http://irep.iium.edu.my/14380/ http://irep.iium.edu.my/14380/ http://irep.iium.edu.my/14380/1/AJB-Rashid_et_al.pdf
first_indexed	2023-09-18T20:23:34Z
last_indexed	2023-09-18T20:23:34Z
_version_	1777408267027218432

Development of pretreatment of empty fruit bunches for enhanced enzymatic saccharification

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