Recovery of recombinant bromelain from Escherichia coli BL21-AI

In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of brom...

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Main Authors:	Bala, M., Salleh, Hamzah Mohd., Amid, Azura, Mel, Maizirwan, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/1/Bala_et_al.pdf

id	iium-14293
recordtype	eprints
spelling	iium-142932012-03-05T06:28:34Z http://irep.iium.edu.my/14293/ Recovery of recombinant bromelain from Escherichia coli BL21-AI Bala, M. Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi T Technology (General) In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant bromelain was expressed as soluble (active) and insoluble (inactive) enzyme in Escherichia coli BL21-A1. The enzyme fractions were purified using Ni-NTA His•Bind resin with the aid of an automated fast protein liquid chromatography (FPLC) system. Purification fold and percentage yield of the purified active bromelain were found to be 3.7 and 64%, respectively. SDS-PAGE results showed that the enzyme was purified to near homogeneity and the soluble form exhibited a single band with molecular weight of about 45 kDa. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14293/1/Bala_et_al.pdf Bala, M. and Salleh, Hamzah Mohd. and Amid, Azura and Mel, Maizirwan and Jami, Mohammed Saedi (2011) Recovery of recombinant bromelain from Escherichia coli BL21-AI. African Journal of Biotechnology, 10 (81). 18829-18832. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Bala%20et%20al.pdf 10.5897/AJB11.2761
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Bala, M. Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi Recovery of recombinant bromelain from Escherichia coli BL21-AI
description	In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant bromelain was expressed as soluble (active) and insoluble (inactive) enzyme in Escherichia coli BL21-A1. The enzyme fractions were purified using Ni-NTA His•Bind resin with the aid of an automated fast protein liquid chromatography (FPLC) system. Purification fold and percentage yield of the purified active bromelain were found to be 3.7 and 64%, respectively. SDS-PAGE results showed that the enzyme was purified to near homogeneity and the soluble form exhibited a single band with molecular weight of about 45 kDa.
format	Article
author	Bala, M. Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi
author_facet	Bala, M. Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi
author_sort	Bala, M.
title	Recovery of recombinant bromelain from Escherichia coli BL21-AI
title_short	Recovery of recombinant bromelain from Escherichia coli BL21-AI
title_full	Recovery of recombinant bromelain from Escherichia coli BL21-AI
title_fullStr	Recovery of recombinant bromelain from Escherichia coli BL21-AI
title_full_unstemmed	Recovery of recombinant bromelain from Escherichia coli BL21-AI
title_sort	recovery of recombinant bromelain from escherichia coli bl21-ai
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/1/Bala_et_al.pdf
first_indexed	2023-09-18T20:23:27Z
last_indexed	2023-09-18T20:23:27Z
_version_	1777408260550164480

Recovery of recombinant bromelain from Escherichia coli BL21-AI

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