Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system

Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protei...

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Main Authors:	Packeer Mohamed, Vasila, Hashim, Yumi Zuhanis Has-Yun, Amid, Azura, Mel, Maizirwan, Kamarulzaman, Abdul Razak, Abdul Wahab, Muhammad Adil, Mohmad Saberi, Salfarina Ezrina
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/14207/ http://irep.iium.edu.my/14207/ http://irep.iium.edu.my/14207/ http://irep.iium.edu.my/14207/1/Mohamed_et_al_1.pdf

id	iium-14207
recordtype	eprints
spelling	iium-142072013-07-02T01:20:01Z http://irep.iium.edu.my/14207/ Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina TP248.13 Biotechnology Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protein from host cells into the culture media is hypothesized to enable reduction or removable of serum from culture media, thus reducing cost. This study was set to investigate the IGF-1 gene expression in Chinese hamster ovary (CHO-K1) cells. The cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose, lactate, total protein and biomass) was done. PCR-based method was performed to study the expression of IGF-1 gene. To this end, it was confirmed that CHO-K1 cells used in this study expressed IGF-1 gene. The study also provides the baseline data on kinetics and biochemical responses of CHO-K1 cell growth. Together, the data would be particularly useful for further studies using CHO-K1 cells as efficient mammalian cell culture host system to produce biologics. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14207/1/Mohamed_et_al_1.pdf Packeer Mohamed, Vasila and Hashim, Yumi Zuhanis Has-Yun and Amid, Azura and Mel, Maizirwan and Kamarulzaman, Abdul Razak and Abdul Wahab, Muhammad Adil and Mohmad Saberi, Salfarina Ezrina (2011) Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system. African Journal of Biotechnology, 10 (81). pp. 18716-18721. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Mohamed%20et%20al%201.pdf 10.5897/AJB11.274
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
description	Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protein from host cells into the culture media is hypothesized to enable reduction or removable of serum from culture media, thus reducing cost. This study was set to investigate the IGF-1 gene expression in Chinese hamster ovary (CHO-K1) cells. The cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose, lactate, total protein and biomass) was done. PCR-based method was performed to study the expression of IGF-1 gene. To this end, it was confirmed that CHO-K1 cells used in this study expressed IGF-1 gene. The study also provides the baseline data on kinetics and biochemical responses of CHO-K1 cell growth. Together, the data would be particularly useful for further studies using CHO-K1 cells as efficient mammalian cell culture host system to produce biologics.
format	Article
author	Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina
author_facet	Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Razak Abdul Wahab, Muhammad Adil Mohmad Saberi, Salfarina Ezrina
author_sort	Packeer Mohamed, Vasila
title	Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
title_short	Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
title_full	Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
title_fullStr	Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
title_full_unstemmed	Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
title_sort	chinese hamster ovary (cho-k1) cells expressed native insulin-like growth factor-1 (igf-1) gene towards efficient mammalian cell culture host system
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/14207/ http://irep.iium.edu.my/14207/ http://irep.iium.edu.my/14207/ http://irep.iium.edu.my/14207/1/Mohamed_et_al_1.pdf
first_indexed	2023-09-18T20:23:23Z
last_indexed	2023-09-18T20:23:23Z
_version_	1777408256365297664

Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system

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