Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum

Calophyllum canum is a large tree which grows in South East Asia and which is popular for its timber. This plant belongs to the family Guttiferae; a family that boasts species which are rich in bioactive phytochemicals. The present study was conducted to screen for the antiproliferation activity of...

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Main Authors:	Bakhtiar, M. Taher, Arief Ichwan, Solachuddin Jauhari, Alkhamaiseh, Suhaib Ibrahim, Suilam Ngoto, Shahida
Format:	Monograph
Language:	English
Published:	[s.n.] 2010
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/14152/ http://irep.iium.edu.my/14152/1/FULL_PROJECT_REPORT.docx

id	iium-14152
recordtype	eprints
spelling	iium-141522014-12-09T01:45:51Z http://irep.iium.edu.my/14152/ Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Alkhamaiseh, Suhaib Ibrahim Suilam Ngoto, Shahida RS Pharmacy and materia medica Calophyllum canum is a large tree which grows in South East Asia and which is popular for its timber. This plant belongs to the family Guttiferae; a family that boasts species which are rich in bioactive phytochemicals. The present study was conducted to screen for the antiproliferation activity of three crude extracts from the stem bark of C. canum. The activity of an isolated compound against canacer cells was also reported. The three extracts; n-hexane, ethyl acetate and methanol extracts, were screened on human cervical (C33A), colon (CACO2), lung (A549), esophageal (TE1), breast (MCF7) cancer cell lines along with normal human dermal fibroblast cells (NHDF) and leukemia cancer cells (K562) . The MTT assay and direct counting by tryphan blue were used to assess the antiproliferation activity of the extracts on the cell lines. The study revealed that the n-hexane extract had the strongest antiproliferation activity, followed by the methanol extract. n-hexane strongly inhibited the growth of TE1 and MCF7 cell lines. IC50 for n-hexane and methanol extract activity on the A549 cell line was found to be 27.96 µg/mL and 78.9 µg/mL respectively. The IC50 for the n-hexane extract activity on the NHDF cell line was found to be 70.59 µg/mL. The ethyl acetate extract showed no activity against any of the cell lines. An isolated compound labeled as CE2 was found to be active against K562 cells with IC50 value of 11µg/mL. [s.n.] 2010-11-30 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/14152/1/FULL_PROJECT_REPORT.docx Bakhtiar, M. Taher and Arief Ichwan, Solachuddin Jauhari and Alkhamaiseh, Suhaib Ibrahim and Suilam Ngoto, Shahida (2010) Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum. Project Report. [s.n.]. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Alkhamaiseh, Suhaib Ibrahim Suilam Ngoto, Shahida Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum
description	Calophyllum canum is a large tree which grows in South East Asia and which is popular for its timber. This plant belongs to the family Guttiferae; a family that boasts species which are rich in bioactive phytochemicals. The present study was conducted to screen for the antiproliferation activity of three crude extracts from the stem bark of C. canum. The activity of an isolated compound against canacer cells was also reported. The three extracts; n-hexane, ethyl acetate and methanol extracts, were screened on human cervical (C33A), colon (CACO2), lung (A549), esophageal (TE1), breast (MCF7) cancer cell lines along with normal human dermal fibroblast cells (NHDF) and leukemia cancer cells (K562) . The MTT assay and direct counting by tryphan blue were used to assess the antiproliferation activity of the extracts on the cell lines. The study revealed that the n-hexane extract had the strongest antiproliferation activity, followed by the methanol extract. n-hexane strongly inhibited the growth of TE1 and MCF7 cell lines. IC50 for n-hexane and methanol extract activity on the A549 cell line was found to be 27.96 µg/mL and 78.9 µg/mL respectively. The IC50 for the n-hexane extract activity on the NHDF cell line was found to be 70.59 µg/mL. The ethyl acetate extract showed no activity against any of the cell lines. An isolated compound labeled as CE2 was found to be active against K562 cells with IC50 value of 11µg/mL.
format	Monograph
author	Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Alkhamaiseh, Suhaib Ibrahim Suilam Ngoto, Shahida
author_facet	Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Alkhamaiseh, Suhaib Ibrahim Suilam Ngoto, Shahida
author_sort	Bakhtiar, M. Taher
title	Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum
title_short	Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum
title_full	Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum
title_fullStr	Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum
title_full_unstemmed	Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum
title_sort	isolation and cytotoxic activity study of phytochemicals from calophyllum canum
publisher	[s.n.]
publishDate	2010
url	http://irep.iium.edu.my/14152/ http://irep.iium.edu.my/14152/1/FULL_PROJECT_REPORT.docx
first_indexed	2023-09-18T20:23:19Z
last_indexed	2023-09-18T20:23:19Z
_version_	1777408252055650304

Isolation and cytotoxic activity study of phytochemicals from Calophyllum canum

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