Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)

Previously, tomato leaves were proved to be one of the potential anticancer agents. High performanceliquid chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation were used to predict the presence of group of metabolites and to ascertain the possibility of certain a...

Full description

Bibliographic Details
Main Authors:	Wan Chik, Wan Dalila, Amid, Azura, Jamal, Parveen
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/13993/ http://irep.iium.edu.my/13993/ http://irep.iium.edu.my/13993/ http://irep.iium.edu.my/13993/1/Chik_et_al.pdf

id	iium-13993
recordtype	eprints
spelling	iium-139932013-07-02T08:24:39Z http://irep.iium.edu.my/13993/ Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR) Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen QR Microbiology Previously, tomato leaves were proved to be one of the potential anticancer agents. High performanceliquid chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation were used to predict the presence of group of metabolites and to ascertain the possibility of certain absorption bands, in which most of the peaks in partially purified tomato (Solanum lycopersicon)leaves methanol extract are attributed to the specific functional groups. The extraction was carried outin a shake flask with 82% methanol, 1:10 (w/v) sample to solvent ratio, agitated at 22°C with 110 rpm within 24 h. Later, the extract was partially purified by column chromatography. HPLC was used to quantify the number of unknown component presents in the fraction. Then, a FT-IR Bruker Tensor 27 System was used during FTIR data acquisition. The collection of FTIR spectra was carried out at 16 scans with resolution of 4 cm -1 using strong apodization in the frequency regions of 4,000 to 650 cm-1.The results support the premise that HPLC and FTIR spectroscopy are efficient and accurate methods for determining major and minor components presents in the extract. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/13993/1/Chik_et_al.pdf Wan Chik, Wan Dalila and Amid, Azura and Jamal, Parveen (2011) Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR). African Journal of Biotechnology, 10 (81). pp. 18666-18673. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Chik%20et%20al.pdf 10.5897/AJB11.2736
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
description	Previously, tomato leaves were proved to be one of the potential anticancer agents. High performanceliquid chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation were used to predict the presence of group of metabolites and to ascertain the possibility of certain absorption bands, in which most of the peaks in partially purified tomato (Solanum lycopersicon)leaves methanol extract are attributed to the specific functional groups. The extraction was carried outin a shake flask with 82% methanol, 1:10 (w/v) sample to solvent ratio, agitated at 22°C with 110 rpm within 24 h. Later, the extract was partially purified by column chromatography. HPLC was used to quantify the number of unknown component presents in the fraction. Then, a FT-IR Bruker Tensor 27 System was used during FTIR data acquisition. The collection of FTIR spectra was carried out at 16 scans with resolution of 4 cm -1 using strong apodization in the frequency regions of 4,000 to 650 cm-1.The results support the premise that HPLC and FTIR spectroscopy are efficient and accurate methods for determining major and minor components presents in the extract.
format	Article
author	Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen
author_facet	Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen
author_sort	Wan Chik, Wan Dalila
title	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_short	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_full	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_fullStr	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_full_unstemmed	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)
title_sort	predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (hplc) and fourier transform infrared (ftir)
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/13993/ http://irep.iium.edu.my/13993/ http://irep.iium.edu.my/13993/ http://irep.iium.edu.my/13993/1/Chik_et_al.pdf
first_indexed	2023-09-18T20:23:11Z
last_indexed	2023-09-18T20:23:11Z
_version_	1777408242921504768

Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by high performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR)

Similar Items