Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa

Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and �,�-diphenyl-�-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture...

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Main Authors:	Amid, Azura, Johan, Nurul Najwa, Jamal, Parveen, Wan Mohd Zain, Wan Norzaidani
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/13962/ http://irep.iium.edu.my/13962/ http://irep.iium.edu.my/13962/ http://irep.iium.edu.my/13962/1/Amid_et_al.pdf

id	iium-13962
recordtype	eprints
spelling	iium-139622013-07-03T01:53:15Z http://irep.iium.edu.my/13962/ Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani T Technology (General) Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and �,�-diphenyl-�-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of J. gendarussa. Callus was induced from green and matured leaves of J. gendarussa cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and benzylaminopurine (BAP). Callus induced on NAA showed higher phenolic content and antioxidant activity as compared to the one induced on 2,4-D. The highest total phenolic content (88 mg GAE/g) was observed in callus culture induced with NAA and BAP, while the highest antioxidant activity (lowest IC50; 8.46 × 10-3mg/ml) was observed in the cell suspension. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/13962/1/Amid_et_al.pdf Amid, Azura and Johan, Nurul Najwa and Jamal, Parveen and Wan Mohd Zain, Wan Norzaidani (2011) Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa. African Journal of Biotechnology, 10 (81). pp. 18653-18656. ISSN ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Amid%20et%20al.pdf 10.5897/AJB11.273
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa
description	Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and �,�-diphenyl-�-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of J. gendarussa. Callus was induced from green and matured leaves of J. gendarussa cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and benzylaminopurine (BAP). Callus induced on NAA showed higher phenolic content and antioxidant activity as compared to the one induced on 2,4-D. The highest total phenolic content (88 mg GAE/g) was observed in callus culture induced with NAA and BAP, while the highest antioxidant activity (lowest IC50; 8.46 × 10-3mg/ml) was observed in the cell suspension.
format	Article
author	Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani
author_facet	Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani
author_sort	Amid, Azura
title	Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa
title_short	Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa
title_full	Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa
title_fullStr	Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa
title_full_unstemmed	Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa
title_sort	observation of antioxidant activity of leaves, callus and suspension culture of justicia gendarusa
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/13962/ http://irep.iium.edu.my/13962/ http://irep.iium.edu.my/13962/ http://irep.iium.edu.my/13962/1/Amid_et_al.pdf
first_indexed	2023-09-18T20:23:09Z
last_indexed	2023-09-18T20:23:09Z
_version_	1777408241021485056

Observation of antioxidant activity of leaves, callus and suspension culture of Justicia gendarusa

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