Phytochemistry and antioxidant activity of garcinia prainiana

Phytochemical investigation of the leaves and stem bark of G. prainiana was carried out. The leaves and stem barks of G. prainiana collected from Kuantan, Pahang were extracted by soxhletextractorwith hexane, dichloromethane and methanol to get the crude exkacts. The crude extracts were purified by...

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Main Authors:	On, Shamsul, Ahmad, Farediah, Mohd Sirat, Hasnah, Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/13932/ http://irep.iium.edu.my/13932/ http://irep.iium.edu.my/13932/4/irep.iium.edu.my_13932_1_ICNP_SHAMSUL_SYAZWANI_13932.pdf

id	iium-13932
recordtype	eprints
spelling	iium-139322014-12-09T01:29:37Z http://irep.iium.edu.my/13932/ Phytochemistry and antioxidant activity of garcinia prainiana On, Shamsul Ahmad, Farediah Mohd Sirat, Hasnah Bakhtiar, M. Taher RS Pharmacy and materia medica Phytochemical investigation of the leaves and stem bark of G. prainiana was carried out. The leaves and stem barks of G. prainiana collected from Kuantan, Pahang were extracted by soxhletextractorwith hexane, dichloromethane and methanol to get the crude exkacts. The crude extracts were purified by column chromatography io yield pure compounds which were characterized spectroscopically using lR, NMR (1D and 2D), UV and MS. Fractionation and purification of the hexane extract of leaves yielded triterpenes, squalene and friedelin. The methanol extract of leaves and stem barks afforded bioflavonoid characterized as morelloflavone and O-methyl fukugetin. Total phenolic content studies were carried out on the crude extracts by using Folin-Ciocalteau reagent. The methanol extract of leaves and stem barks showed the highest total phenolic content expressed as gallic acid and (t)-catechin equivalents. These extracts also showed the highest value of ascorbic acid and butylated hydroxytoluene (BHT) equivalents on forming the phosphomolybdenum complex in the total antioxidant assay. The antioxidant assay on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical showed that the methanolextract of stem barks had the highest free radical scavenging activity with lCso value 74.5trr9/ml while the isolated compound, morelloflavone revealed a strong free radical scavenging activity with lCsovalue 15.7p9/ml 2011-11-14 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/13932/4/irep.iium.edu.my_13932_1_ICNP_SHAMSUL_SYAZWANI_13932.pdf On, Shamsul and Ahmad, Farediah and Mohd Sirat, Hasnah and Bakhtiar, M. Taher (2011) Phytochemistry and antioxidant activity of garcinia prainiana. In: International Conference on Natural products (ICNP 2011), 14-16 November 2011, Palm garden Resort, Putrajaya. http://www.icnp2011.upm.edu.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica On, Shamsul Ahmad, Farediah Mohd Sirat, Hasnah Bakhtiar, M. Taher Phytochemistry and antioxidant activity of garcinia prainiana
description	Phytochemical investigation of the leaves and stem bark of G. prainiana was carried out. The leaves and stem barks of G. prainiana collected from Kuantan, Pahang were extracted by soxhletextractorwith hexane, dichloromethane and methanol to get the crude exkacts. The crude extracts were purified by column chromatography io yield pure compounds which were characterized spectroscopically using lR, NMR (1D and 2D), UV and MS. Fractionation and purification of the hexane extract of leaves yielded triterpenes, squalene and friedelin. The methanol extract of leaves and stem barks afforded bioflavonoid characterized as morelloflavone and O-methyl fukugetin. Total phenolic content studies were carried out on the crude extracts by using Folin-Ciocalteau reagent. The methanol extract of leaves and stem barks showed the highest total phenolic content expressed as gallic acid and (t)-catechin equivalents. These extracts also showed the highest value of ascorbic acid and butylated hydroxytoluene (BHT) equivalents on forming the phosphomolybdenum complex in the total antioxidant assay. The antioxidant assay on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical showed that the methanolextract of stem barks had the highest free radical scavenging activity with lCso value 74.5trr9/ml while the isolated compound, morelloflavone revealed a strong free radical scavenging activity with lCsovalue 15.7p9/ml
format	Conference or Workshop Item
author	On, Shamsul Ahmad, Farediah Mohd Sirat, Hasnah Bakhtiar, M. Taher
author_facet	On, Shamsul Ahmad, Farediah Mohd Sirat, Hasnah Bakhtiar, M. Taher
author_sort	On, Shamsul
title	Phytochemistry and antioxidant activity of garcinia prainiana
title_short	Phytochemistry and antioxidant activity of garcinia prainiana
title_full	Phytochemistry and antioxidant activity of garcinia prainiana
title_fullStr	Phytochemistry and antioxidant activity of garcinia prainiana
title_full_unstemmed	Phytochemistry and antioxidant activity of garcinia prainiana
title_sort	phytochemistry and antioxidant activity of garcinia prainiana
publishDate	2011
url	http://irep.iium.edu.my/13932/ http://irep.iium.edu.my/13932/ http://irep.iium.edu.my/13932/4/irep.iium.edu.my_13932_1_ICNP_SHAMSUL_SYAZWANI_13932.pdf
first_indexed	2023-09-18T20:23:07Z
last_indexed	2023-09-18T20:23:07Z
_version_	1777408238928527360

Phytochemistry and antioxidant activity of garcinia prainiana

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