Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases

Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viabi...

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Main Authors:	W. S. , Wan Muhammad Salahudin, Tun, Ye, Zulkifly, Ahmad Hafiz
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QM Human anatomy
Online Access:	http://irep.iium.edu.my/13782/ http://irep.iium.edu.my/13782/1/Histological_%26_Histochemical_studies_of_LL_muscles_in_Diabetis_Mellitus_cases.pdf

id	iium-13782
recordtype	eprints
spelling	iium-137822017-06-21T06:55:26Z http://irep.iium.edu.my/13782/ Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz QM Human anatomy Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viability of muscle and subsequently stump healing. Aim of the study: To describe the fiber size and oxidative capacity quantitatively in 3 muscles of the leg Materials and methods: Specimen obtained from the 10 diabetes mellitus subjects undergone below knee amputation (BKA). Specimen was stained for enzyme histochemistry NADH-TR staining. The slide was analyzed with ImageJ software for fiber type distribution and oxidative capacity based on staining density. Result and discussion: Gastrocnemius muscle has predominantly Type 1 muscle fibers and highest oxidative capacity, while the anterior tibialis muscle has predominantly Type 2 muscle fibers and lowest oxidative capacity. Soleus muscle has almost equal Type 1 and 2 muscle fibers and has intermediate oxidative capacity. Conclusion: Muscle with predominant type 1 fibers has highest oxidative capacity. 2011-12-21 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/13782/1/Histological_%26_Histochemical_studies_of_LL_muscles_in_Diabetis_Mellitus_cases.pdf W. S. , Wan Muhammad Salahudin and Tun, Ye and Zulkifly, Ahmad Hafiz (2011) Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases. In: 20th Scientific Conference of the Microscopy Society of Malaysia & 21st Annual General Meeting, 20-22 December 2011, Port Disckson, Negeri Sembilan. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QM Human anatomy
spellingShingle	QM Human anatomy W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
description	Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viability of muscle and subsequently stump healing. Aim of the study: To describe the fiber size and oxidative capacity quantitatively in 3 muscles of the leg Materials and methods: Specimen obtained from the 10 diabetes mellitus subjects undergone below knee amputation (BKA). Specimen was stained for enzyme histochemistry NADH-TR staining. The slide was analyzed with ImageJ software for fiber type distribution and oxidative capacity based on staining density. Result and discussion: Gastrocnemius muscle has predominantly Type 1 muscle fibers and highest oxidative capacity, while the anterior tibialis muscle has predominantly Type 2 muscle fibers and lowest oxidative capacity. Soleus muscle has almost equal Type 1 and 2 muscle fibers and has intermediate oxidative capacity. Conclusion: Muscle with predominant type 1 fibers has highest oxidative capacity.
format	Conference or Workshop Item
author	W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz
author_facet	W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz
author_sort	W. S. , Wan Muhammad Salahudin
title	Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
title_short	Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
title_full	Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
title_fullStr	Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
title_full_unstemmed	Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
title_sort	quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
publishDate	2011
url	http://irep.iium.edu.my/13782/ http://irep.iium.edu.my/13782/1/Histological_%26_Histochemical_studies_of_LL_muscles_in_Diabetis_Mellitus_cases.pdf
first_indexed	2023-09-18T20:22:56Z
last_indexed	2023-09-18T20:22:56Z
_version_	1777408228110368768

Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases

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