Optimization of Newcastle disease virus production in T-flask
Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/1/Arifin_et_al.pdf |
| Summary: | Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry.
Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the
disadvantages of being labour-intensive, time consuming and requires large area for the incubation of
eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by
using cell culture method was studied. Experiments were carried out in T-flasks to investigate the
effects of serum concentration in the culture medium during virus replication phase and multiplicity of
infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x
107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used |
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