Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation

The brain’s hippocampus which controls memory and learning is the first structure affected by neurodegeneration in Alzheimer’s disease (AD). The number of viable hippocampal CA1 pyramidal cells (HCA1PC) is significantly decreased in AD and in rats with 2 vessel occlusion (2VO), an established animal...

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Main Authors:	Azzubaidi, Marwan Saad, Saxena, Anil Kumar, Talib, Norlelawati A.
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Online Access:	http://irep.iium.edu.my/13515/ http://irep.iium.edu.my/13515/ http://irep.iium.edu.my/13515/1/Electron_Microscopy_Conf._2011.docx

id	iium-13515
recordtype	eprints
spelling	iium-135152012-12-17T06:23:13Z http://irep.iium.edu.my/13515/ Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation Azzubaidi, Marwan Saad Saxena, Anil Kumar Talib, Norlelawati A. RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry The brain’s hippocampus which controls memory and learning is the first structure affected by neurodegeneration in Alzheimer’s disease (AD). The number of viable hippocampal CA1 pyramidal cells (HCA1PC) is significantly decreased in AD and in rats with 2 vessel occlusion (2VO), an established animal model for AD. The variability in three dimensional arrangements of HCA1PC between dorsal and ventral portions of the hippocampus made the calculation of HCA1PC subject to debate. As errors and biases in the counting technique can be overcome by implementing a standardized procedure, the current study proposed a procedure for quantification of the viable HCA1PC by standardizing the method of tissue sectioning. Eight rats were divided into 2VO and sham control with four animals per group. Coronal sections were made based on the anatomical landmarks of medial section of rat’s cerebral hemispheres. Under light microscope with cresyl violet staining, the number of viable HCA1PC cells in 1 mm horizontal distance of dorsal hippocampus, 3 mm caudal to the fornix, was calculated twice and found to be significantly higher in sham control than 2VO group (p<0.01) with standard error of mean of 4.98 and 17.26 respectively. This indicates successful establishment of the standardized technique with feasible reproducibility. 1 International Islamic University Malaysia, Faculty of Medicine Department of Basic Medical Sciences 2011-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/13515/1/Electron_Microscopy_Conf._2011.docx Azzubaidi, Marwan Saad and Saxena, Anil Kumar and Talib, Norlelawati A. (2011) Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation. In: Harnessing Microscopy and Nuclear Science for Green Technology, 20-22 December 2011, Port Dickson, Malaysia. http://www.anilkumar@iium.edu.com.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
spellingShingle	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Azzubaidi, Marwan Saad Saxena, Anil Kumar Talib, Norlelawati A. Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation
description	The brain’s hippocampus which controls memory and learning is the first structure affected by neurodegeneration in Alzheimer’s disease (AD). The number of viable hippocampal CA1 pyramidal cells (HCA1PC) is significantly decreased in AD and in rats with 2 vessel occlusion (2VO), an established animal model for AD. The variability in three dimensional arrangements of HCA1PC between dorsal and ventral portions of the hippocampus made the calculation of HCA1PC subject to debate. As errors and biases in the counting technique can be overcome by implementing a standardized procedure, the current study proposed a procedure for quantification of the viable HCA1PC by standardizing the method of tissue sectioning. Eight rats were divided into 2VO and sham control with four animals per group. Coronal sections were made based on the anatomical landmarks of medial section of rat’s cerebral hemispheres. Under light microscope with cresyl violet staining, the number of viable HCA1PC cells in 1 mm horizontal distance of dorsal hippocampus, 3 mm caudal to the fornix, was calculated twice and found to be significantly higher in sham control than 2VO group (p<0.01) with standard error of mean of 4.98 and 17.26 respectively. This indicates successful establishment of the standardized technique with feasible reproducibility. 1 International Islamic University Malaysia, Faculty of Medicine Department of Basic Medical Sciences
format	Conference or Workshop Item
author	Azzubaidi, Marwan Saad Saxena, Anil Kumar Talib, Norlelawati A.
author_facet	Azzubaidi, Marwan Saad Saxena, Anil Kumar Talib, Norlelawati A.
author_sort	Azzubaidi, Marwan Saad
title	Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation
title_short	Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation
title_full	Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation
title_fullStr	Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation
title_full_unstemmed	Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation
title_sort	quantifying dorsal hippocampal ca1 pyramidal cells in rats: rules to light microscope based estimation
publishDate	2011
url	http://irep.iium.edu.my/13515/ http://irep.iium.edu.my/13515/ http://irep.iium.edu.my/13515/1/Electron_Microscopy_Conf._2011.docx
first_indexed	2023-09-18T20:22:41Z
last_indexed	2023-09-18T20:22:41Z
_version_	1777408211930841088

Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation

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