Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats

Introduction: A growing body of evidence now supports the critically attained threshold cerebral hypoperfusion (CATCH) hypothesis for triggering age related neurodegeneration. Experimentally, such threshold is achieved in rats by permanent bilateral common carotid artery ligation (PBCCAL). The hip...

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Main Author:	Ahmed, Qamar Uddin
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/13355/ http://irep.iium.edu.my/13355/2/IIUM_2011_Internation_conference_participation.pdf

id	iium-13355
recordtype	eprints
spelling	iium-133552012-02-02T08:27:15Z http://irep.iium.edu.my/13355/ Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats Ahmed, Qamar Uddin RM300 Drugs and their action Introduction: A growing body of evidence now supports the critically attained threshold cerebral hypoperfusion (CATCH) hypothesis for triggering age related neurodegeneration. Experimentally, such threshold is achieved in rats by permanent bilateral common carotid artery ligation (PBCCAL). The hippocampal CA1 region pyramidal cells are the earliest neurons to degenerate following this procedure. Nigella sativa oil extract (NSO) was found to preserve viability of cerebellar neuron cells in vitro putting forward its potential neuroprotective effect. Objective: To assess the effect of NSO on hippocampal pyramidal cells of rats with CATCH that was achieved through PBCCAL procedure. Methodology: 30 rats were equally divided into three groups: sham control, untreated PBCCAL and NSO treated group (PBCCAL with daily oral NSO treatment). After the 10th postoperative week coronal sections of the hippocampus were stained with cresyl violet and Fluoro Jade-C (FJC) stains. Results: Cresyl violet staining demonstrated significantly higher number of viable pyramidal cells within CA1 hippocampal region in sham control (p<0.001), and NSO (p<0.001) treated groups as compared to the untreated PBCCAL group while the difference was not significant when comparing the viable pyramidal cells number of sham control with NSO treated groups (p=0.568). FJC staining did not display any difference in fluorescence intensity among study groups. Conclusion: NSO has the potential to protect hippocampal pyramidal cells from neurodegeneration. Further conclusive studies are encouraged to confirm the beneficial effect of NSO in preventing age related neurodegeneration namely Alzheimer’s disease and its subsequent cognitive impairment. 2011-12-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/13355/2/IIUM_2011_Internation_conference_participation.pdf Ahmed, Qamar Uddin (2011) Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats. In: International Health Conference IIUM 2011 , 7 - 8 December 2011, Kuantan, Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Ahmed, Qamar Uddin Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats
description	Introduction: A growing body of evidence now supports the critically attained threshold cerebral hypoperfusion (CATCH) hypothesis for triggering age related neurodegeneration. Experimentally, such threshold is achieved in rats by permanent bilateral common carotid artery ligation (PBCCAL). The hippocampal CA1 region pyramidal cells are the earliest neurons to degenerate following this procedure. Nigella sativa oil extract (NSO) was found to preserve viability of cerebellar neuron cells in vitro putting forward its potential neuroprotective effect. Objective: To assess the effect of NSO on hippocampal pyramidal cells of rats with CATCH that was achieved through PBCCAL procedure. Methodology: 30 rats were equally divided into three groups: sham control, untreated PBCCAL and NSO treated group (PBCCAL with daily oral NSO treatment). After the 10th postoperative week coronal sections of the hippocampus were stained with cresyl violet and Fluoro Jade-C (FJC) stains. Results: Cresyl violet staining demonstrated significantly higher number of viable pyramidal cells within CA1 hippocampal region in sham control (p<0.001), and NSO (p<0.001) treated groups as compared to the untreated PBCCAL group while the difference was not significant when comparing the viable pyramidal cells number of sham control with NSO treated groups (p=0.568). FJC staining did not display any difference in fluorescence intensity among study groups. Conclusion: NSO has the potential to protect hippocampal pyramidal cells from neurodegeneration. Further conclusive studies are encouraged to confirm the beneficial effect of NSO in preventing age related neurodegeneration namely Alzheimer’s disease and its subsequent cognitive impairment.
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin
author_facet	Ahmed, Qamar Uddin
author_sort	Ahmed, Qamar Uddin
title	Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats
title_short	Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats
title_full	Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats
title_fullStr	Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats
title_full_unstemmed	Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats
title_sort	preventive effect of nigella sativa oil against hippocampal pyramidal cell loss induced by permanent bilateral common carotid artey occlusion in rats
publishDate	2011
url	http://irep.iium.edu.my/13355/ http://irep.iium.edu.my/13355/2/IIUM_2011_Internation_conference_participation.pdf
first_indexed	2023-09-18T20:22:32Z
last_indexed	2023-09-18T20:22:32Z
_version_	1777408202707566592

Preventive effect of Nigella Sativa oil against Hippocampal Pyramidal Cell loss induced by permanent bilateral common Carotid Artey Occlusion in rats

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