HCV genotypes in haemodialysis units: a preliminary study

Introduction: Serological verification among haemodialysis patients in Kuantan, Pahang, an East Coast town in Peninsular Malaysia is routinely updated for every 3 months by Ministry of Health. However, the determination of the HCV genotypes for each infected patient has not yet been attempted. This...

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Main Authors:	Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Abdullah, Nasuruddin
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/13281/ http://irep.iium.edu.my/13281/ http://irep.iium.edu.my/13281/1/PIIS1201971208003603.pdf

id	iium-13281
recordtype	eprints
spelling	iium-132812013-07-03T08:29:14Z http://irep.iium.edu.my/13281/ HCV genotypes in haemodialysis units: a preliminary study Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin QR Microbiology Introduction: Serological verification among haemodialysis patients in Kuantan, Pahang, an East Coast town in Peninsular Malaysia is routinely updated for every 3 months by Ministry of Health. However, the determination of the HCV genotypes for each infected patient has not yet been attempted. This study aims at the determination of HCV genotypes among infected haemodialysis patients. Methodology: 22 seropositive patients out of 208 (10.5%) from 4 heamodialysis units (HDUs) were enrolled in this study during the period from March to August 2007. Detection of HCV RNA from serum was done by using RT-PCR technique targeting 212 bp of the 5’UTR region. Meanwhile, the base sequences of the above regions were deduced using the same primers as for the RT-PCR. The nucleotide sequences data were analyzed with Bioedit software and classification of the genotypes was done using neighbor-joining method together with known sequences obtained from NCBI homepage. Result: Out of 21 samples (male = 57%), 2 are negatives. The 19 positive HCV-RNA samples were subjected for sequencing analysis. The analysis showed that 63.2% (12/19) were of genotype 3, 26.3% (5/19) of genotype 1 followed by 5.3% (1/19) of genotype 4 and 1 isolate (5.3%) is undetermined. Furthermore, the genotype distribution showed discrete clustering among units. Sequence comparisons revealed that 4 strains from one HDU have a G insertion (-208) which is not detected in other strains including ones that were retrieved from NCBI sequence database. Conclusion: Genotype 3 is the most prevalent genotype found among heamodialysis patients in Kuantan which is in concordance with previously published HCV genotype prevalence in Malaysia. Furthermore, genotype clustering suggests a nosocomial and blood transfusion infection which requires further subtyping test. Meanwhile, nucleotide sequence analysis suggested that HCV adaptation to certain environment may lead to mutations in the well conserved region (5’UTR). 2008-06-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/13281/1/PIIS1201971208003603.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah, Nasuruddin (2008) HCV genotypes in haemodialysis units: a preliminary study. In: 13th International Congress On Infectious Diseases, 19th-22nd June 2008, Kuala Lumpur Convention Centre, Kuala Lumpur. http://ww2.isid.org/Downloads/13th_ICID_ProgFriday.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin HCV genotypes in haemodialysis units: a preliminary study
description	Introduction: Serological verification among haemodialysis patients in Kuantan, Pahang, an East Coast town in Peninsular Malaysia is routinely updated for every 3 months by Ministry of Health. However, the determination of the HCV genotypes for each infected patient has not yet been attempted. This study aims at the determination of HCV genotypes among infected haemodialysis patients. Methodology: 22 seropositive patients out of 208 (10.5%) from 4 heamodialysis units (HDUs) were enrolled in this study during the period from March to August 2007. Detection of HCV RNA from serum was done by using RT-PCR technique targeting 212 bp of the 5’UTR region. Meanwhile, the base sequences of the above regions were deduced using the same primers as for the RT-PCR. The nucleotide sequences data were analyzed with Bioedit software and classification of the genotypes was done using neighbor-joining method together with known sequences obtained from NCBI homepage. Result: Out of 21 samples (male = 57%), 2 are negatives. The 19 positive HCV-RNA samples were subjected for sequencing analysis. The analysis showed that 63.2% (12/19) were of genotype 3, 26.3% (5/19) of genotype 1 followed by 5.3% (1/19) of genotype 4 and 1 isolate (5.3%) is undetermined. Furthermore, the genotype distribution showed discrete clustering among units. Sequence comparisons revealed that 4 strains from one HDU have a G insertion (-208) which is not detected in other strains including ones that were retrieved from NCBI sequence database. Conclusion: Genotype 3 is the most prevalent genotype found among heamodialysis patients in Kuantan which is in concordance with previously published HCV genotype prevalence in Malaysia. Furthermore, genotype clustering suggests a nosocomial and blood transfusion infection which requires further subtyping test. Meanwhile, nucleotide sequence analysis suggested that HCV adaptation to certain environment may lead to mutations in the well conserved region (5’UTR).
format	Conference or Workshop Item
author	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin
author_facet	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin
author_sort	Hamzah, Hairul Aini
title	HCV genotypes in haemodialysis units: a preliminary study
title_short	HCV genotypes in haemodialysis units: a preliminary study
title_full	HCV genotypes in haemodialysis units: a preliminary study
title_fullStr	HCV genotypes in haemodialysis units: a preliminary study
title_full_unstemmed	HCV genotypes in haemodialysis units: a preliminary study
title_sort	hcv genotypes in haemodialysis units: a preliminary study
publishDate	2008
url	http://irep.iium.edu.my/13281/ http://irep.iium.edu.my/13281/ http://irep.iium.edu.my/13281/1/PIIS1201971208003603.pdf
first_indexed	2023-09-18T20:22:27Z
last_indexed	2023-09-18T20:22:27Z
_version_	1777408197768773632

HCV genotypes in haemodialysis units: a preliminary study

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