Histomorphometric analysis of the endothelial cell in skin microvasculature among diabetic foot ulcer patients
Changes affecting endothelial cells in the microcirculation of diabetic foot patients are important in the development of foot ulceration and subsequent failure to heal existing ulcers. Methods We studied 30 patients (10 control individuals, 10 with the neuropathic ulcer and 10 with neuroischa...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/13270/ http://irep.iium.edu.my/13270/1/poster_emsm.pdf |
| Summary: | Changes affecting endothelial cells in the microcirculation of diabetic foot patients are important in the development of foot ulceration and subsequent failure to heal existing ulcers.
Methods
We studied 30 patients (10 control individuals, 10 with the neuropathic ulcer and 10 with neuroischaemic ulcer). The specimens were taken using skin punch biopsy and were routinely processed for light microscopy. The mean endothelial cell count (ECC) was performed by calculating all the endothelial cells of the 3 selected arterioles in each slide of every patient. The mean endothelial cell thicknesses (ECT) of all endothelia were measured using the image analyzer.
Results
There were significant differences of ECC and ECT between the groups determined by one-way ANOVA (F(2,27) = 5.31, p = 0.01 and F(2,27) = 52.42, p < 0.01 respectivel)y. There was no significant differences of ECC and ECT between neuropathic and neuroischemic; p = 0.99 and p = 0.18 respectively.
Conclusion
The decrement of ECC and the increment of ECT among diabetic foot patients as compared to controls could be due to the alteration in the endothelial cell function and structure as the results of the metabolic disease. |
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