Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences

This study aims to determine the phylogenetic relationship between Holothuria leucospilota (Echinodermata: Holothuroidea) from Malaysia and other Holothuria species using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. Holothuria (Mertensiothuria) leucospilota (Bran...

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Main Authors:	Idris, Mohd Yaman, Ahmad, Hajar Fauzan, Hashim, Ridzwan, Usup, Gires, Kamarudin, Kamarul Rahim, Mohamed Rehan, Aisyah
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/12938/ http://irep.iium.edu.my/12938/ http://irep.iium.edu.my/12938/1/2010_NCEH_Mohd_Yaman_et_al_Mac.pdf

id	iium-12938
recordtype	eprints
spelling	iium-129382012-02-02T12:50:37Z http://irep.iium.edu.my/12938/ Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences Idris, Mohd Yaman Ahmad, Hajar Fauzan Hashim, Ridzwan Usup, Gires Kamarudin, Kamarul Rahim Mohamed Rehan, Aisyah QL Zoology This study aims to determine the phylogenetic relationship between Holothuria leucospilota (Echinodermata: Holothuroidea) from Malaysia and other Holothuria species using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or locally known as bat puntil is currently the most abundant sea cucumber species in Malaysia. This coral reef species is not in danger of extinction like commercial Stichopus species that are locally known as gamat such as Stichopus horrens. Previously, COI mtDNA gene sequences of H. leucospilota were unavailable online in GenBank, National Center for Biotechnology Information (NCBI); thus leading to this phylogenetic study. In this study, specimens of H. leucospilota were collected from Intan Besar Island, Langkawi, Malaysia. Eight COI mtDNA gene sequences of H. leucospilota obtained from this study have been registered with the GenBank (Accession no.: FJ223873 - FJ223880, submission date: 19 September 2008). Three main methods namely neighbour joining, maximum parsimony and maximum likelihood were used for phylogenetic tree reconstruction. Present results showed that H. leucospilota was genetically closer to H. coluber as compared to H. atra. Even so, further studies with more samples, broad geographical sampling and various mitochondrial DNA genes are necessary to obtain better information and verification on molecular phylogeny of H. leucospilota in particular and Holothuria species in general. 2010-03-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/12938/1/2010_NCEH_Mohd_Yaman_et_al_Mac.pdf Idris, Mohd Yaman and Ahmad, Hajar Fauzan and Hashim, Ridzwan and Usup, Gires and Kamarudin, Kamarul Rahim and Mohamed Rehan, Aisyah (2010) Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences. In: 2nd National Conference on Environment & Health 2010, 17 - 18 March 2010, Renaissance Hotel, Kota Bharu, Kelantan Darul Naim, Malaysia. http://www.ppsk.usm.my/seminar/nceh2010.nsf/posterAbstract
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology
spellingShingle	QL Zoology Idris, Mohd Yaman Ahmad, Hajar Fauzan Hashim, Ridzwan Usup, Gires Kamarudin, Kamarul Rahim Mohamed Rehan, Aisyah Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences
description	This study aims to determine the phylogenetic relationship between Holothuria leucospilota (Echinodermata: Holothuroidea) from Malaysia and other Holothuria species using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or locally known as bat puntil is currently the most abundant sea cucumber species in Malaysia. This coral reef species is not in danger of extinction like commercial Stichopus species that are locally known as gamat such as Stichopus horrens. Previously, COI mtDNA gene sequences of H. leucospilota were unavailable online in GenBank, National Center for Biotechnology Information (NCBI); thus leading to this phylogenetic study. In this study, specimens of H. leucospilota were collected from Intan Besar Island, Langkawi, Malaysia. Eight COI mtDNA gene sequences of H. leucospilota obtained from this study have been registered with the GenBank (Accession no.: FJ223873 - FJ223880, submission date: 19 September 2008). Three main methods namely neighbour joining, maximum parsimony and maximum likelihood were used for phylogenetic tree reconstruction. Present results showed that H. leucospilota was genetically closer to H. coluber as compared to H. atra. Even so, further studies with more samples, broad geographical sampling and various mitochondrial DNA genes are necessary to obtain better information and verification on molecular phylogeny of H. leucospilota in particular and Holothuria species in general.
format	Conference or Workshop Item
author	Idris, Mohd Yaman Ahmad, Hajar Fauzan Hashim, Ridzwan Usup, Gires Kamarudin, Kamarul Rahim Mohamed Rehan, Aisyah
author_facet	Idris, Mohd Yaman Ahmad, Hajar Fauzan Hashim, Ridzwan Usup, Gires Kamarudin, Kamarul Rahim Mohamed Rehan, Aisyah
author_sort	Idris, Mohd Yaman
title	Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences
title_short	Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences
title_full	Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences
title_fullStr	Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences
title_full_unstemmed	Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences
title_sort	phylogeny of holothuria leucospilota (echinodermata: holothuroidea) as inferred from cytochrome c oxidase i gene sequences
publishDate	2010
url	http://irep.iium.edu.my/12938/ http://irep.iium.edu.my/12938/ http://irep.iium.edu.my/12938/1/2010_NCEH_Mohd_Yaman_et_al_Mac.pdf
first_indexed	2023-09-18T20:22:04Z
last_indexed	2023-09-18T20:22:04Z
_version_	1777408172803227648

Phylogeny of Holothuria leucospilota (Echinodermata: Holothuroidea) as inferred from cytochrome c oxidase I gene sequences

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