Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA

Diverse species of sea cucumber (Echinodermata: Holothuroidea) were documented in Malaysia. Their abundance benefits the Malaysians in traditional and modern medicinal industry as well as in food processing industry. Previously, the identification of sea cucumber species in Malaysia was done mainl...

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Main Authors:	Hussin, Rosnah, Kamarudin, Kamarul Rahim, Nordin, Noor Faizul Hadry, Hashim, Ridzwan, Mohammad , Mohd Nahar
Format:	Conference or Workshop Item
Language:	English English English
Published:	2006
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/12936/ http://irep.iium.edu.my/12936/5/NCSTIE_2006_Acceptance_Letter.pdf http://irep.iium.edu.my/12936/1/2006_NCSTIE_Rosnah_Kamarul.pdf http://irep.iium.edu.my/12936/4/NCSTIE_2006_programme_book.pdf

id	iium-12936
recordtype	eprints
spelling	iium-129362013-08-30T02:25:01Z http://irep.iium.edu.my/12936/ Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA Hussin, Rosnah Kamarudin, Kamarul Rahim Nordin, Noor Faizul Hadry Hashim, Ridzwan Mohammad , Mohd Nahar QL Zoology Diverse species of sea cucumber (Echinodermata: Holothuroidea) were documented in Malaysia. Their abundance benefits the Malaysians in traditional and modern medicinal industry as well as in food processing industry. Previously, the identification of sea cucumber species in Malaysia was done mainly based on morphological characters. Among the characters used were the shapes of ossicles, body shapes and colour, shapes of tentacles, the existence and shapes of papillae. However, the morphological approaches were unsuccessfully unravelled the problematic taxonomic status of sea cucumbers as there are unidentified and unknown species present throughout Malaysia to date. As an alternative approach to verify the species status inferred from morphology, molecular ecology analyses were incorporated in this study to examine the phylogenetic relationship between and among selected species of sea cucumbers from Malaysia by using cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. Three genera were selected namely Holothuria and Stichopus from order Aspidochirotida, and genus Molpadia from order Molpadiida. Polymerase Chain Reaction (PCR) gave the desired products of amplified COI mtDNA region with length of approximately 510 bp. After the multiple sequence alignment, partial sequences of COI mtDNA gene ranging from 488 bp to 489 bp were utilized for further analyses. 22 ingroups of sea cucumbers and one outgroup represented by Diadema setosum were included in the reconstruction of neighbour-joining (NJ) tree and maximum parsimony (MP) tree. Base frequency and genetic distance based on Kimura-2 parameter model were also calculated prior to the reconstruction of phylogenetic tree. Basically, both types of phylogenetic trees suggest the paraphyly of Holothuria and Stichopus due to the clustering of four Stichopus horrens individuals together with Holothuria species. Apart from that, Molpadia species seems genetically close to the main group of genus Holothuria and Stichopus horrens. The phylogenetic results were also supported by the data from genetic distance. Overall, the above findings strongly suggest that the grouping and classification at species and higher level as inferred from COI mtDNA gene are unresolved, requiring further molecular ecology studies to be done in the near future. 2006-12-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/12936/5/NCSTIE_2006_Acceptance_Letter.pdf application/pdf en http://irep.iium.edu.my/12936/1/2006_NCSTIE_Rosnah_Kamarul.pdf application/pdf en http://irep.iium.edu.my/12936/4/NCSTIE_2006_programme_book.pdf Hussin, Rosnah and Kamarudin, Kamarul Rahim and Nordin, Noor Faizul Hadry and Hashim, Ridzwan and Mohammad , Mohd Nahar (2006) Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA. In: International Conference on Science & Technology: Applications in Industry & Education , 8 - 9 December 2006, Universiti Teknologi MARA, Pulau Pinang, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QL Zoology
spellingShingle	QL Zoology Hussin, Rosnah Kamarudin, Kamarul Rahim Nordin, Noor Faizul Hadry Hashim, Ridzwan Mohammad , Mohd Nahar Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA
description	Diverse species of sea cucumber (Echinodermata: Holothuroidea) were documented in Malaysia. Their abundance benefits the Malaysians in traditional and modern medicinal industry as well as in food processing industry. Previously, the identification of sea cucumber species in Malaysia was done mainly based on morphological characters. Among the characters used were the shapes of ossicles, body shapes and colour, shapes of tentacles, the existence and shapes of papillae. However, the morphological approaches were unsuccessfully unravelled the problematic taxonomic status of sea cucumbers as there are unidentified and unknown species present throughout Malaysia to date. As an alternative approach to verify the species status inferred from morphology, molecular ecology analyses were incorporated in this study to examine the phylogenetic relationship between and among selected species of sea cucumbers from Malaysia by using cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. Three genera were selected namely Holothuria and Stichopus from order Aspidochirotida, and genus Molpadia from order Molpadiida. Polymerase Chain Reaction (PCR) gave the desired products of amplified COI mtDNA region with length of approximately 510 bp. After the multiple sequence alignment, partial sequences of COI mtDNA gene ranging from 488 bp to 489 bp were utilized for further analyses. 22 ingroups of sea cucumbers and one outgroup represented by Diadema setosum were included in the reconstruction of neighbour-joining (NJ) tree and maximum parsimony (MP) tree. Base frequency and genetic distance based on Kimura-2 parameter model were also calculated prior to the reconstruction of phylogenetic tree. Basically, both types of phylogenetic trees suggest the paraphyly of Holothuria and Stichopus due to the clustering of four Stichopus horrens individuals together with Holothuria species. Apart from that, Molpadia species seems genetically close to the main group of genus Holothuria and Stichopus horrens. The phylogenetic results were also supported by the data from genetic distance. Overall, the above findings strongly suggest that the grouping and classification at species and higher level as inferred from COI mtDNA gene are unresolved, requiring further molecular ecology studies to be done in the near future.
format	Conference or Workshop Item
author	Hussin, Rosnah Kamarudin, Kamarul Rahim Nordin, Noor Faizul Hadry Hashim, Ridzwan Mohammad , Mohd Nahar
author_facet	Hussin, Rosnah Kamarudin, Kamarul Rahim Nordin, Noor Faizul Hadry Hashim, Ridzwan Mohammad , Mohd Nahar
author_sort	Hussin, Rosnah
title	Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA
title_short	Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA
title_full	Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA
title_fullStr	Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA
title_full_unstemmed	Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA
title_sort	phylogenetic analysis of sea cucumbers (echinodermata: holothuroidea) in malaysia as inferred from cytochrome c oxidase i mtdna
publishDate	2006
url	http://irep.iium.edu.my/12936/ http://irep.iium.edu.my/12936/5/NCSTIE_2006_Acceptance_Letter.pdf http://irep.iium.edu.my/12936/1/2006_NCSTIE_Rosnah_Kamarul.pdf http://irep.iium.edu.my/12936/4/NCSTIE_2006_programme_book.pdf
first_indexed	2023-09-18T20:22:04Z
last_indexed	2023-09-18T20:22:04Z
_version_	1777408172652232704

Phylogenetic analysis of sea cucumbers (Echinodermata: Holothuroidea) in Malaysia as inferred from Cytochrome C Oxidase I MtDNA

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