Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model

Objective. The aim of this study was to determine if autologous bone marrow mesenchymal stem cells (AMSCs) cultured in chondrogenic medium containing growth factors could repair meniscus tissue and retard surgically induced osteoarthritis in the knee joint. Methods. Autologous Sheep bone marrow st...

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Main Authors:	Alfaqeh, Hamoud Hussein Mohammed, Chua, Kienhui, MY , Norhamdan, HC, Chen, BS , Aminuddin, BHI , Ruszymah
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	QP Physiology
Online Access:	http://irep.iium.edu.my/12734/ http://irep.iium.edu.my/12734/1/Intraarticular_injection_of_autologous_chondrogenic_induced_bone.pdf

id	iium-12734
recordtype	eprints
spelling	iium-127342015-12-04T08:23:22Z http://irep.iium.edu.my/12734/ Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model Alfaqeh, Hamoud Hussein Mohammed Chua, Kienhui MY , Norhamdan HC, Chen BS , Aminuddin BHI , Ruszymah QP Physiology Objective. The aim of this study was to determine if autologous bone marrow mesenchymal stem cells (AMSCs) cultured in chondrogenic medium containing growth factors could repair meniscus tissue and retard surgically induced osteoarthritis in the knee joint. Methods. Autologous Sheep bone marrow stem cells (AMSCs) were aspirated from sheep iliac crest and cultured in medium containing 5ng/ml TGFβ3 + 50ng/ml IGF-1 for 3 weeks. Six weeks after surgically induced osteoarthritis; a single dose of chondrogenic inducted AMSCs suspended in 5 ml Dulbecco’s Modified Eagle Medium (DMEM) at density of 10 million cells was injected intra-articularly into the osteoarthritic knee joint. After addition of another 6 weeks, the sheep were scarified and the femoral condyle and the proximal tibia were removed, fixed and photographed. The gross of the removed knee joint were graded by a blinded assessor based on ICRS grading system. Part of the tissue was imbedded in paraffin and sectioned into 5µm and stained with H&E and Safranin O. Result. The results indicated that surgically induced osteoarthritic knee joint treated with chondrogenic inducted AMSCs showed clear evidence of minimum articular cartilage destruction with meniscus repaired when compared with other control knee joints. Conclusion Our findings demonstrated that intra-articular injection of chondrogenic inducted AMSCs could stimulate regeneration of meniscal tissue and retards the progressive of osteoarthritis in sheep model. 2009-09-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/12734/1/Intraarticular_injection_of_autologous_chondrogenic_induced_bone.pdf Alfaqeh, Hamoud Hussein Mohammed and Chua, Kienhui and MY , Norhamdan and HC, Chen and BS , Aminuddin and BHI , Ruszymah (2009) Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model. In: International Conference of Tissue Engineering and Regenerative Medicine, 31 Aug-3 Sep 2009, Seoul, South Korea.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Alfaqeh, Hamoud Hussein Mohammed Chua, Kienhui MY , Norhamdan HC, Chen BS , Aminuddin BHI , Ruszymah Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
description	Objective. The aim of this study was to determine if autologous bone marrow mesenchymal stem cells (AMSCs) cultured in chondrogenic medium containing growth factors could repair meniscus tissue and retard surgically induced osteoarthritis in the knee joint. Methods. Autologous Sheep bone marrow stem cells (AMSCs) were aspirated from sheep iliac crest and cultured in medium containing 5ng/ml TGFβ3 + 50ng/ml IGF-1 for 3 weeks. Six weeks after surgically induced osteoarthritis; a single dose of chondrogenic inducted AMSCs suspended in 5 ml Dulbecco’s Modified Eagle Medium (DMEM) at density of 10 million cells was injected intra-articularly into the osteoarthritic knee joint. After addition of another 6 weeks, the sheep were scarified and the femoral condyle and the proximal tibia were removed, fixed and photographed. The gross of the removed knee joint were graded by a blinded assessor based on ICRS grading system. Part of the tissue was imbedded in paraffin and sectioned into 5µm and stained with H&E and Safranin O. Result. The results indicated that surgically induced osteoarthritic knee joint treated with chondrogenic inducted AMSCs showed clear evidence of minimum articular cartilage destruction with meniscus repaired when compared with other control knee joints. Conclusion Our findings demonstrated that intra-articular injection of chondrogenic inducted AMSCs could stimulate regeneration of meniscal tissue and retards the progressive of osteoarthritis in sheep model.
format	Conference or Workshop Item
author	Alfaqeh, Hamoud Hussein Mohammed Chua, Kienhui MY , Norhamdan HC, Chen BS , Aminuddin BHI , Ruszymah
author_facet	Alfaqeh, Hamoud Hussein Mohammed Chua, Kienhui MY , Norhamdan HC, Chen BS , Aminuddin BHI , Ruszymah
author_sort	Alfaqeh, Hamoud Hussein Mohammed
title	Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
title_short	Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
title_full	Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
title_fullStr	Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
title_full_unstemmed	Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
title_sort	intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model
publishDate	2009
url	http://irep.iium.edu.my/12734/ http://irep.iium.edu.my/12734/1/Intraarticular_injection_of_autologous_chondrogenic_induced_bone.pdf
first_indexed	2023-09-18T20:21:54Z
last_indexed	2023-09-18T20:21:54Z
_version_	1777408162282864640

Intraarticular injection of autologous chondrogenic induced bone marrow stem cells promotes regenesis of traumatic osteoarthritis and promotes repair of meniscus injury in a sheep model

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