Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment

Abstract: Objectives: This study was aimed to examine the effect of two different media on bone marrow stem cells (BMSCs) chondrogenesis in vitro and in vivo. Methods: BMSCs were isolated from sheep and divided into two test groups and control group. Control group was cultured in medium supple...

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Main Authors:	Alfaqeh, Hamoud Hussein Mohammed, Kienhui, Chua, Bin Saim, Aminuddin, Bint Haji Idus, Ruszymah
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	QP Physiology
Online Access:	http://irep.iium.edu.my/12551/ http://irep.iium.edu.my/12551/1/Nude_mice_-_poster_presentation.pdf

id	iium-12551
recordtype	eprints
spelling	iium-125512012-12-27T06:11:39Z http://irep.iium.edu.my/12551/ Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment Alfaqeh, Hamoud Hussein Mohammed Kienhui, Chua Bin Saim, Aminuddin Bint Haji Idus, Ruszymah QP Physiology Abstract: Objectives: This study was aimed to examine the effect of two different media on bone marrow stem cells (BMSCs) chondrogenesis in vitro and in vivo. Methods: BMSCs were isolated from sheep and divided into two test groups and control group. Control group was cultured in medium supplemented with 10 % fetal bovine serum (FBS). Test group A was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL IGF-1, 3ng/mL bFGF and 1ng/mL TGF-β2. Test group B was cultured in medium supplemented with 1%FBS, 1% ITS, 5ng/mL TGF-β3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out in Institute of Medical Research (IMR) and Universiti Kebangsaan Medical Centre Malaysia, Kuala Lumpur city, from 19 April 2007 to 25 December 2008. Results: Cell aggregates were formed in test group A and more in test group B denoted the early chondrogenesis in culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 weeks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group B constructs showed highest cartilage matrix synthesis confirmed by Safranin O staining and GAG production. Conclusion: These results demonstrated the effectiveness of medium supplemented with TGF-β3 in the combination to promote chondrogenesis in BMSCs. 2008-07-23 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/12551/1/Nude_mice_-_poster_presentation.pdf Alfaqeh, Hamoud Hussein Mohammed and Kienhui, Chua and Bin Saim, Aminuddin and Bint Haji Idus, Ruszymah (2008) Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment. In: 2nd Malaysia Tissue Engineering and Regenerative Medicine 22-23 July 2008 , 22-23 July 2008, HUKM .
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Alfaqeh, Hamoud Hussein Mohammed Kienhui, Chua Bin Saim, Aminuddin Bint Haji Idus, Ruszymah Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
description	Abstract: Objectives: This study was aimed to examine the effect of two different media on bone marrow stem cells (BMSCs) chondrogenesis in vitro and in vivo. Methods: BMSCs were isolated from sheep and divided into two test groups and control group. Control group was cultured in medium supplemented with 10 % fetal bovine serum (FBS). Test group A was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL IGF-1, 3ng/mL bFGF and 1ng/mL TGF-β2. Test group B was cultured in medium supplemented with 1%FBS, 1% ITS, 5ng/mL TGF-β3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out in Institute of Medical Research (IMR) and Universiti Kebangsaan Medical Centre Malaysia, Kuala Lumpur city, from 19 April 2007 to 25 December 2008. Results: Cell aggregates were formed in test group A and more in test group B denoted the early chondrogenesis in culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 weeks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group B constructs showed highest cartilage matrix synthesis confirmed by Safranin O staining and GAG production. Conclusion: These results demonstrated the effectiveness of medium supplemented with TGF-β3 in the combination to promote chondrogenesis in BMSCs.
format	Conference or Workshop Item
author	Alfaqeh, Hamoud Hussein Mohammed Kienhui, Chua Bin Saim, Aminuddin Bint Haji Idus, Ruszymah
author_facet	Alfaqeh, Hamoud Hussein Mohammed Kienhui, Chua Bin Saim, Aminuddin Bint Haji Idus, Ruszymah
author_sort	Alfaqeh, Hamoud Hussein Mohammed
title	Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
title_short	Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
title_full	Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
title_fullStr	Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
title_full_unstemmed	Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
title_sort	effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment
publishDate	2008
url	http://irep.iium.edu.my/12551/ http://irep.iium.edu.my/12551/1/Nude_mice_-_poster_presentation.pdf
first_indexed	2023-09-18T20:21:44Z
last_indexed	2023-09-18T20:21:44Z
_version_	1777408151791861760

Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment

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