Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes

Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes

The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mous...

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Main Authors: Rahman, Mohammad Tariqur, De Ley, Marc
Format: Article
Language:English
Published: Springer Netherlands 2008
Subjects:
QH301 Biology
Online Access:http://irep.iium.edu.my/12469/
http://irep.iium.edu.my/12469/
http://irep.iium.edu.my/12469/
http://irep.iium.edu.my/12469/1/fulltext.pdf
id iium-12469
recordtype eprints
spelling iium-124692012-12-06T23:54:10Z http://irep.iium.edu.my/12469/ Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes Rahman, Mohammad Tariqur De Ley, Marc QH301 Biology The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h cell populations left untreated with zinc. Springer Netherlands 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/12469/1/fulltext.pdf Rahman, Mohammad Tariqur and De Ley, Marc (2008) Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes. Cell biology and toxicology, 24 (1). pp. 19-25. ISSN 0742-2091 (P), 1573-6822 (O) http://www.springerlink.com/content/997227111149j0h6/?MUD=MP 10.1007/s10565-007-9012-3
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QH301 Biology
spellingShingle QH301 Biology
Rahman, Mohammad Tariqur
De Ley, Marc
Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
description The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h cell populations left untreated with zinc.
format Article
author Rahman, Mohammad Tariqur
De Ley, Marc
author_facet Rahman, Mohammad Tariqur
De Ley, Marc
author_sort Rahman, Mohammad Tariqur
title Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
title_short Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
title_full Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
title_fullStr Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
title_full_unstemmed Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
title_sort metallothionein in human thrombocyte precursors, cd61+ megakaryocytes
publisher Springer Netherlands
publishDate 2008
url http://irep.iium.edu.my/12469/
http://irep.iium.edu.my/12469/
http://irep.iium.edu.my/12469/
http://irep.iium.edu.my/12469/1/fulltext.pdf
first_indexed 2023-09-18T20:21:41Z
last_indexed 2023-09-18T20:21:41Z
_version_ 1777408148656619520

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