Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mous...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Netherlands
2008
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/1/fulltext.pdf |
| Summary: | The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h cell populations left untreated with zinc. |
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