Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua

Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua

This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-l...

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Main Authors: Qaralleh, Haitham, Syed Osman Idid, Syed Zahir Idid, Saad, Shahbudin, Darnis, Deny Susanti
Format: Conference or Workshop Item
Language:English
English
Published: 2010
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/12158/
http://irep.iium.edu.my/12158/
http://irep.iium.edu.my/12158/1/Icastcover.pdf
http://irep.iium.edu.my/12158/5/isolation_of_antibacterial_compounds_from_dichloromethane_fraction_of_Malaysian_sponges_Neopetrosiaexigua.pdf
id iium-12158
recordtype eprints
spelling iium-121582012-01-17T05:12:49Z http://irep.iium.edu.my/12158/ Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua Qaralleh, Haitham Syed Osman Idid, Syed Zahir Idid Saad, Shahbudin Darnis, Deny Susanti Q Science (General) This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-liquid extraction was performed using solvenants with different polarity (n-hexane, carbon tetrachloride, dicholoromathane, n-butanol and water in order to isolate the active antibacterial compounds. Bassed on the biological guided fractionation results, dichloromathane and n-butanol fractions showed the hisgest antimicrobial activity. Purification of dichloromathane fraction was done by using SephadexLH-20, normal phase column chromatographyand reverse phase column chromatography to yeild three compounds (1-3). All compounds were tested for their antibacterial activity against Staphyloccus aureus. The results showed that 3 exhibited significant antibacterial activity. Research is still in progress to elucidate the structures of the islated compounds. 2010-11-27 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/12158/1/Icastcover.pdf application/pdf en http://irep.iium.edu.my/12158/5/isolation_of_antibacterial_compounds_from_dichloromethane_fraction_of_Malaysian_sponges_Neopetrosiaexigua.pdf Qaralleh, Haitham and Syed Osman Idid, Syed Zahir Idid and Saad, Shahbudin and Darnis, Deny Susanti (2010) Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Malaysia. http://www.iium.edu.my/icast/2010/upload/3rd%20IIUM-iCAST%202010%20Abstract%20Book.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Qaralleh, Haitham
Syed Osman Idid, Syed Zahir Idid
Saad, Shahbudin
Darnis, Deny Susanti
Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
description This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-liquid extraction was performed using solvenants with different polarity (n-hexane, carbon tetrachloride, dicholoromathane, n-butanol and water in order to isolate the active antibacterial compounds. Bassed on the biological guided fractionation results, dichloromathane and n-butanol fractions showed the hisgest antimicrobial activity. Purification of dichloromathane fraction was done by using SephadexLH-20, normal phase column chromatographyand reverse phase column chromatography to yeild three compounds (1-3). All compounds were tested for their antibacterial activity against Staphyloccus aureus. The results showed that 3 exhibited significant antibacterial activity. Research is still in progress to elucidate the structures of the islated compounds.
format Conference or Workshop Item
author Qaralleh, Haitham
Syed Osman Idid, Syed Zahir Idid
Saad, Shahbudin
Darnis, Deny Susanti
author_facet Qaralleh, Haitham
Syed Osman Idid, Syed Zahir Idid
Saad, Shahbudin
Darnis, Deny Susanti
author_sort Qaralleh, Haitham
title Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
title_short Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
title_full Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
title_fullStr Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
title_full_unstemmed Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua
title_sort isolation of antibacterial compounds from dichloromethane fraction of malaysian sponges neopetrosiaexigua
publishDate 2010
url http://irep.iium.edu.my/12158/
http://irep.iium.edu.my/12158/
http://irep.iium.edu.my/12158/1/Icastcover.pdf
http://irep.iium.edu.my/12158/5/isolation_of_antibacterial_compounds_from_dichloromethane_fraction_of_Malaysian_sponges_Neopetrosiaexigua.pdf
first_indexed 2023-09-18T20:21:24Z
last_indexed 2023-09-18T20:21:24Z
_version_ 1777408131365601280

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