Effect of Malaysian anti-diabetic plant extracts on adipocyte cells

Diabetic mellitus (DM)is a disease of worldwide significance and increasing prevalence. It is a metabolic disorder characterized by a predisposition to developing significantly raised blood glucose. The objective of this research was to evaluate the antidiabetic properties of medicinal plants ued in...

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Main Authors:	Ali, Mohamed Zaffar, Bakhtiar, M. Taher, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English
Published:	2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/12151/ http://irep.iium.edu.my/12151/1/Icastcover.pdf http://irep.iium.edu.my/12151/2/Icast3.pdf

id	iium-12151
recordtype	eprints
spelling	iium-121512014-12-09T01:37:43Z http://irep.iium.edu.my/12151/ Effect of Malaysian anti-diabetic plant extracts on adipocyte cells Ali, Mohamed Zaffar Bakhtiar, M. Taher Darnis, Deny Susanti Q Science (General) Diabetic mellitus (DM)is a disease of worldwide significance and increasing prevalence. It is a metabolic disorder characterized by a predisposition to developing significantly raised blood glucose. The objective of this research was to evaluate the antidiabetic properties of medicinal plants ued in the treatment if type 2 diabetes. The selection of the plants was based on traditional reputation of usefulness in treating diabetes.Ethanolic extracts of Andrographis paniculata, Cinnamomum zeylanicum, Lagerstroemia speciosa, Momodica charantia, Orthosiphon stamineus, Phyllanthus amarus, Pithecellobium jiringa, Syzgium polyanthum, and Tinospora cirspa were selected. Insulin was used as a postive control. The plants were studied on the bioactive peptides (adipokines) using an in vitro model. Cells were seeded and counted everyday for ten days. The first step was to test the ability of the plants to induce predipocytes to adipocyte.cells. Prior to the testing, preadipocytes (3T3-L1) cells were differentiated to adipocytes by using a mixture of dexamethasone, isobutlymethylxanthine and the plant extract. Treated cells were stained by usingg oil red ) and the absorbance was read at 340 nm. 3T3-L1 preadipocytes exhibited the maximum growth at day-four(4.7x105 cells. Oil red O staining results showed that C.zeylanicum, L.speciosa leaves and S.polyanthum extracts have strong activity in inducing lipid formation and the others have moderate properties. The results of the study suggest the validiy of the plants for clinical usein treatmentof type 2 diabetes, after their toxicological investigation. This finding may lead to seriousresearch towardsa developing new and efficient drugs for diabetes. 2010-11-27 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/12151/1/Icastcover.pdf application/pdf en http://irep.iium.edu.my/12151/2/Icast3.pdf Ali, Mohamed Zaffar and Bakhtiar, M. Taher and Darnis, Deny Susanti (2010) Effect of Malaysian anti-diabetic plant extracts on adipocyte cells. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ali, Mohamed Zaffar Bakhtiar, M. Taher Darnis, Deny Susanti Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
description	Diabetic mellitus (DM)is a disease of worldwide significance and increasing prevalence. It is a metabolic disorder characterized by a predisposition to developing significantly raised blood glucose. The objective of this research was to evaluate the antidiabetic properties of medicinal plants ued in the treatment if type 2 diabetes. The selection of the plants was based on traditional reputation of usefulness in treating diabetes.Ethanolic extracts of Andrographis paniculata, Cinnamomum zeylanicum, Lagerstroemia speciosa, Momodica charantia, Orthosiphon stamineus, Phyllanthus amarus, Pithecellobium jiringa, Syzgium polyanthum, and Tinospora cirspa were selected. Insulin was used as a postive control. The plants were studied on the bioactive peptides (adipokines) using an in vitro model. Cells were seeded and counted everyday for ten days. The first step was to test the ability of the plants to induce predipocytes to adipocyte.cells. Prior to the testing, preadipocytes (3T3-L1) cells were differentiated to adipocytes by using a mixture of dexamethasone, isobutlymethylxanthine and the plant extract. Treated cells were stained by usingg oil red ) and the absorbance was read at 340 nm. 3T3-L1 preadipocytes exhibited the maximum growth at day-four(4.7x105 cells. Oil red O staining results showed that C.zeylanicum, L.speciosa leaves and S.polyanthum extracts have strong activity in inducing lipid formation and the others have moderate properties. The results of the study suggest the validiy of the plants for clinical usein treatmentof type 2 diabetes, after their toxicological investigation. This finding may lead to seriousresearch towardsa developing new and efficient drugs for diabetes.
format	Conference or Workshop Item
author	Ali, Mohamed Zaffar Bakhtiar, M. Taher Darnis, Deny Susanti
author_facet	Ali, Mohamed Zaffar Bakhtiar, M. Taher Darnis, Deny Susanti
author_sort	Ali, Mohamed Zaffar
title	Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_short	Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_full	Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_fullStr	Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_full_unstemmed	Effect of Malaysian anti-diabetic plant extracts on adipocyte cells
title_sort	effect of malaysian anti-diabetic plant extracts on adipocyte cells
publishDate	2010
url	http://irep.iium.edu.my/12151/ http://irep.iium.edu.my/12151/1/Icastcover.pdf http://irep.iium.edu.my/12151/2/Icast3.pdf
first_indexed	2023-09-18T20:21:24Z
last_indexed	2023-09-18T20:21:24Z
_version_	1777408130911567872

Effect of Malaysian anti-diabetic plant extracts on adipocyte cells

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