The production of carbon nanotubes using two-stage chemical vapor deposition and their potential use in protein purification
Carbon nanotubes (CNTs) were produced by gas phase two-stage chemical vapor deposition using ferrocene as a catalyst and acetylene (C2H2) and hydrogen (H2) as precursor gases. The CNTs were characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/12095/ http://irep.iium.edu.my/12095/ http://irep.iium.edu.my/12095/ http://irep.iium.edu.my/12095/4/Chemical_engineering_journal-1_%282%29_FY.pdf |
| Summary: | Carbon nanotubes (CNTs) were produced by gas phase two-stage chemical vapor deposition using
ferrocene as a catalyst and acetylene (C2H2) and hydrogen (H2) as precursor gases. The CNTs were characterized
using field emission scanning electron microscopy (FESEM), transmission electron microscopy
(TEM), and by thermogravimetric analysis (TGA). Process parameters such as reaction temperature, reaction
time and gas flow rates of C2H2 and H2 were optimized using Design-Expert software v.6.0.8. The
statistical analysis reveals that the optimum conditions for the CNT production were reaction temperature
of 850 ◦C, reaction time of 60 min and gases flow rates of 40 and 150 ml/min for C2H2 and H2,
respectively. The optimum CNTs prepared were purified and functionalized using acid treatment. Both
functionalized and nonfunctionalized CNTs were used as column chromatographic media for skim latex
protein purification. Skim latex protein was used as the model protein for the recovery of useful proteins
and enzymes. During purification, process parameters such as pH and ionic strength of the running
buffer were optimized to enhance protein purification. Results revealed that CNTs behave efficiently as
a hydrophobic interaction chromatographic (HIC) media.
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