Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts

Sea cucumber is a marine invertebrate of the phylum Echinodermata and the class Holothuroidea that found on the sea floor worldwide. In Asia, many of sea cucumber species are considered as traditional food items and have been used in featment of numerous diseases such as eczema, arthritis and hypert...

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Main Authors:	Althunibat, Osama Yousef, Hashim, Ridzwan, Bakhtiar, M. Taher, Mohd. Daud, Jamaludin, Ikeda, Masa-Aki
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/12033/ http://irep.iium.edu.my/12033/ http://irep.iium.edu.my/12033/1/bandung_osama_2009.pdf

id	iium-12033
recordtype	eprints
spelling	iium-120332017-06-20T07:54:03Z http://irep.iium.edu.my/12033/ Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki RS Pharmacy and materia medica Sea cucumber is a marine invertebrate of the phylum Echinodermata and the class Holothuroidea that found on the sea floor worldwide. In Asia, many of sea cucumber species are considered as traditional food items and have been used in featment of numerous diseases such as eczema, arthritis and hypertension. Previous scientific studies have shown multiple biological activities of sea cucumber species as antinociceptive, antimicrobial and antifungal properties. This study was conducted to investigate the antioxidant and antiproliferative activities of aqueous and organic extracts from sea cucumber, Holothuria edulis. Two different free radical systems were used to evaluate antioxidant activity of H. edulis, stable radical l,l-diphenyl-2- picrylhydrazyl (DPPH.) and linoleic acid free radical mediated p-carotene bleaching. In addition, Folin-Ciocalteau reagent was used to determine the total phenolic content of the exfacts. The inhibitory effect of the exfacts on proliferation of MCF-7 (human breast adenocarcinoma) and TEI (Human esophageal squamous cell carcinoma) human cancer cell lines were demonstrated by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our data showed that the gallic acid equivalent (GAE) of the total phenolic content in aqueous extract (GAE : 7.33 mglg) is higher than an organic extact (GAE = 2.17 mglg). An aqueous extract also exhibited higher.antioxidant capacity by using DPPH assay (ICso = 2.04 mg/ml vs. 8.73 mlmL in organic extract), as well as by using p-carotene bleaching assay (Antioxidant Activity : 42.69 %o vs. 28.52 % in organic extract). On the other hand, an organic extact showed higher antiproliferative effect against MCF-7 and TEI cancer cells, giving ICso : 28.0 and 17.5 pdmL, respectively than aqueous extract, that gave ICso : 133.0 pilmL against MCF- '7 and76.0 pgmL against TEl. In conclusion, frndings of this study revealed that H. edulis as a promising source ofnatural antioxidants and anticancer products. 2009 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/12033/1/bandung_osama_2009.pdf Althunibat, Osama Yousef and Hashim, Ridzwan and Bakhtiar, M. Taher and Mohd. Daud, Jamaludin and Ikeda, Masa-Aki (2009) Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts. In: Bandung International Conference on Medicinal Chemistry, 6-8 August 2009, Bandung, Indonesia. http://www.icmc2009.fa.itb.ac.id/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts
description	Sea cucumber is a marine invertebrate of the phylum Echinodermata and the class Holothuroidea that found on the sea floor worldwide. In Asia, many of sea cucumber species are considered as traditional food items and have been used in featment of numerous diseases such as eczema, arthritis and hypertension. Previous scientific studies have shown multiple biological activities of sea cucumber species as antinociceptive, antimicrobial and antifungal properties. This study was conducted to investigate the antioxidant and antiproliferative activities of aqueous and organic extracts from sea cucumber, Holothuria edulis. Two different free radical systems were used to evaluate antioxidant activity of H. edulis, stable radical l,l-diphenyl-2- picrylhydrazyl (DPPH.) and linoleic acid free radical mediated p-carotene bleaching. In addition, Folin-Ciocalteau reagent was used to determine the total phenolic content of the exfacts. The inhibitory effect of the exfacts on proliferation of MCF-7 (human breast adenocarcinoma) and TEI (Human esophageal squamous cell carcinoma) human cancer cell lines were demonstrated by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our data showed that the gallic acid equivalent (GAE) of the total phenolic content in aqueous extract (GAE : 7.33 mglg) is higher than an organic extact (GAE = 2.17 mglg). An aqueous extract also exhibited higher.antioxidant capacity by using DPPH assay (ICso = 2.04 mg/ml vs. 8.73 mlmL in organic extract), as well as by using p-carotene bleaching assay (Antioxidant Activity : 42.69 %o vs. 28.52 % in organic extract). On the other hand, an organic extact showed higher antiproliferative effect against MCF-7 and TEI cancer cells, giving ICso : 28.0 and 17.5 pdmL, respectively than aqueous extract, that gave ICso : 133.0 pilmL against MCF- '7 and76.0 pgmL against TEl. In conclusion, frndings of this study revealed that H. edulis as a promising source ofnatural antioxidants and anticancer products.
format	Conference or Workshop Item
author	Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki
author_facet	Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki
author_sort	Althunibat, Osama Yousef
title	Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts
title_short	Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts
title_full	Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts
title_fullStr	Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts
title_full_unstemmed	Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts
title_sort	antioxidant and antiproliferative activities of malaysian sea cucumber, (holothuria edulis lesson), extracts
publishDate	2009
url	http://irep.iium.edu.my/12033/ http://irep.iium.edu.my/12033/ http://irep.iium.edu.my/12033/1/bandung_osama_2009.pdf
first_indexed	2023-09-18T20:21:15Z
last_indexed	2023-09-18T20:21:15Z
_version_	1777408121600212992

Antioxidant and antiproliferative activities of Malaysian sea cucumber, (Holothuria edulis Lesson), extracts

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