Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang

HCV infection is frequent in patients undergoing maintenance haemodialysis, with prevalence between 8 and 10%. Hepatitis C has an adverse effect on both patient and graft survival in those who get renal transplants. There are relatively scarce reports on the natural fluctuation in viral load level i...

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Main Authors:	Abdul Majid, Mohammed Saad, Mustafa Mahmoud, Mohammed Imad Al-Deen, Hamzah, Hairul Aini, Seman ., M. R
Format:	Article
Language:	English
Published:	The Faculty of Medicine, International Islamic University Malaysia 2012
Subjects:	QR355 Virology R Medicine (General)
Online Access:	http://irep.iium.edu.my/11363/ http://irep.iium.edu.my/11363/ http://irep.iium.edu.my/11363/1/IMJM_Vol11No1-2012-p23-30.pdf

id	iium-11363
recordtype	eprints
spelling	iium-113632013-04-11T03:09:58Z http://irep.iium.edu.my/11363/ Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang Abdul Majid, Mohammed Saad Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini Seman ., M. R QR355 Virology R Medicine (General) HCV infection is frequent in patients undergoing maintenance haemodialysis, with prevalence between 8 and 10%. Hepatitis C has an adverse effect on both patient and graft survival in those who get renal transplants. There are relatively scarce reports on the natural fluctuation in viral load level in patients on chronic haemodialysis. The present study is a longitudinal short-term three months study where 27 chronic haemodialysis patients infected with known HCV genotypes were recruited from seven haemodialysis centres in Pahang. Serum samples were collected monthly, both pre- and post-haemodialysis sessions, over a period of three months. Viral RNA was extracted from serum using QIAamp Viral RNA Extraction kit (Qiagen). The HCV viral load was measured using one step reverse transcriptase qPCR (Applied Biosystems) targeting the 5`HCV non-coding region (5’UTR). The serum α-IFN level was measured using commercial ELISA kit (Amersham, UK). Six biochemical liver function tests (AST, ALP, TP, albumin, ALT and TB) were also done for all pre-haemodialysis samples. All patients showed persistent low level viral load that varied significantly over the study period (P = 0.001). HCV genotype 1 viral load was significantly higher than that of genotype 3. No apparent correlation could be recognized between the viral loads and the corresponding interferon-alpha levels which were detectable in only few patients during the period of study. The Faculty of Medicine, International Islamic University Malaysia 2012-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/11363/1/IMJM_Vol11No1-2012-p23-30.pdf Abdul Majid, Mohammed Saad and Mustafa Mahmoud, Mohammed Imad Al-Deen and Hamzah, Hairul Aini and Seman ., M. R (2012) Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang. The International Medical Journal Malaysia, 11 (1). pp. 23-30. ISSN 1823-4631 http://www.e-imj.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR355 Virology R Medicine (General)
spellingShingle	QR355 Virology R Medicine (General) Abdul Majid, Mohammed Saad Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini Seman ., M. R Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang
description	HCV infection is frequent in patients undergoing maintenance haemodialysis, with prevalence between 8 and 10%. Hepatitis C has an adverse effect on both patient and graft survival in those who get renal transplants. There are relatively scarce reports on the natural fluctuation in viral load level in patients on chronic haemodialysis. The present study is a longitudinal short-term three months study where 27 chronic haemodialysis patients infected with known HCV genotypes were recruited from seven haemodialysis centres in Pahang. Serum samples were collected monthly, both pre- and post-haemodialysis sessions, over a period of three months. Viral RNA was extracted from serum using QIAamp Viral RNA Extraction kit (Qiagen). The HCV viral load was measured using one step reverse transcriptase qPCR (Applied Biosystems) targeting the 5`HCV non-coding region (5’UTR). The serum α-IFN level was measured using commercial ELISA kit (Amersham, UK). Six biochemical liver function tests (AST, ALP, TP, albumin, ALT and TB) were also done for all pre-haemodialysis samples. All patients showed persistent low level viral load that varied significantly over the study period (P = 0.001). HCV genotype 1 viral load was significantly higher than that of genotype 3. No apparent correlation could be recognized between the viral loads and the corresponding interferon-alpha levels which were detectable in only few patients during the period of study.
format	Article
author	Abdul Majid, Mohammed Saad Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini Seman ., M. R
author_facet	Abdul Majid, Mohammed Saad Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini Seman ., M. R
author_sort	Abdul Majid, Mohammed Saad
title	Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang
title_short	Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang
title_full	Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang
title_fullStr	Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang
title_full_unstemmed	Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang
title_sort	biodynamics of hepatitis c virus in haemodialysis patients in pahang
publisher	The Faculty of Medicine, International Islamic University Malaysia
publishDate	2012
url	http://irep.iium.edu.my/11363/ http://irep.iium.edu.my/11363/ http://irep.iium.edu.my/11363/1/IMJM_Vol11No1-2012-p23-30.pdf
first_indexed	2023-09-18T20:20:42Z
last_indexed	2023-09-18T20:20:42Z
_version_	1777408087104159744

Biodynamics of Hepatitis C virus in haemodialysis patients in Pahang

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