Biodynamics of HCV infection in haemodialysis patients in Pahang

Hepatitis C is a global disease, WHO has described it as a “viral time bomb”. In Malaysia, the sero-prevalence is 1.6%. HCV infection is frequent in patients undergoing maintenance haemodialysis, with prevalence between 8 and 10%. Hepatitis C has an adverse effect on both patient and graft survival...

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Main Authors:	Mustafa Mahmoud, Mohammed Imad Al-Deen, Abdul Majid, Mohammed Saad, Hamzah, Hairul Aini, Hasmani, Mohamed Hadzri
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QR355 Virology R Medicine (General)
Online Access:	http://irep.iium.edu.my/11347/ http://irep.iium.edu.my/11347/ http://irep.iium.edu.my/11347/1/IRIE_2011_HCV_Biodynamics.pdf

id	iium-11347
recordtype	eprints
spelling	iium-113472011-12-23T07:49:14Z http://irep.iium.edu.my/11347/ Biodynamics of HCV infection in haemodialysis patients in Pahang Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hamzah, Hairul Aini Hasmani, Mohamed Hadzri QR355 Virology R Medicine (General) Hepatitis C is a global disease, WHO has described it as a “viral time bomb”. In Malaysia, the sero-prevalence is 1.6%. HCV infection is frequent in patients undergoing maintenance haemodialysis, with prevalence between 8 and 10%. Hepatitis C has an adverse effect on both patient and graft survival in those who get renal transplants. There are relatively scarce reports on the natural fluctuation in viral load and alpha interferon (α-IFN) level in patients on chronic hemodialysis. Methods A longitudinal short-term three months study where 27 chronic hemodialysis patients infected with known HCV genotypes were recruited from seven hemodialysis centers in Pahang. Serum samples were collected monthly, both pre- and post-hemodialysis sessions, over a period of three months. Viral RNA was extracted from serum using QIAamp Viral RNA Extraction kit (Qiagen). The HCV viral load was measured using one step reverse transcriptase qPCR (Applied Biosystems) targeting the 5`HCV non-coding region. The serum α-IFN level was measured using commercial ELISA kit (Amersham, UK). Six biochemical liver function tests (AST, ALP, TP, albumin, ALT and TB) were also done for all pre-hemodialysis samples. Results All patients showed persistant low level viral load (Fig.1) that varied significantly over the study period (P = 0.001). HCV genotype 1 viral load was significantly higher than that of genotype 3 (Fig.2). The difference between pre- and post-haemodialysis viral load was statistically insignificant. No significant correlation between viral load and liver function status was noted. No correlation was observed between pre-haemodialysis serum α-IFN level and pre-haemodialysis viral load. The difference between pre and post-haemodialysis plasma α-IFN levels was statistically insignificant. 2011-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/11347/1/IRIE_2011_HCV_Biodynamics.pdf Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdul Majid, Mohammed Saad and Hamzah, Hairul Aini and Hasmani, Mohamed Hadzri (2011) Biodynamics of HCV infection in haemodialysis patients in Pahang. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2011, 9-10 February 2011, Cultural Activity Centre (CAC) and KAED Gallery IIUM. (Unpublished) http://www.iium.edu.my/irie/11/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR355 Virology R Medicine (General)
spellingShingle	QR355 Virology R Medicine (General) Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hamzah, Hairul Aini Hasmani, Mohamed Hadzri Biodynamics of HCV infection in haemodialysis patients in Pahang
description	Hepatitis C is a global disease, WHO has described it as a “viral time bomb”. In Malaysia, the sero-prevalence is 1.6%. HCV infection is frequent in patients undergoing maintenance haemodialysis, with prevalence between 8 and 10%. Hepatitis C has an adverse effect on both patient and graft survival in those who get renal transplants. There are relatively scarce reports on the natural fluctuation in viral load and alpha interferon (α-IFN) level in patients on chronic hemodialysis. Methods A longitudinal short-term three months study where 27 chronic hemodialysis patients infected with known HCV genotypes were recruited from seven hemodialysis centers in Pahang. Serum samples were collected monthly, both pre- and post-hemodialysis sessions, over a period of three months. Viral RNA was extracted from serum using QIAamp Viral RNA Extraction kit (Qiagen). The HCV viral load was measured using one step reverse transcriptase qPCR (Applied Biosystems) targeting the 5`HCV non-coding region. The serum α-IFN level was measured using commercial ELISA kit (Amersham, UK). Six biochemical liver function tests (AST, ALP, TP, albumin, ALT and TB) were also done for all pre-hemodialysis samples. Results All patients showed persistant low level viral load (Fig.1) that varied significantly over the study period (P = 0.001). HCV genotype 1 viral load was significantly higher than that of genotype 3 (Fig.2). The difference between pre- and post-haemodialysis viral load was statistically insignificant. No significant correlation between viral load and liver function status was noted. No correlation was observed between pre-haemodialysis serum α-IFN level and pre-haemodialysis viral load. The difference between pre and post-haemodialysis plasma α-IFN levels was statistically insignificant.
format	Conference or Workshop Item
author	Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hamzah, Hairul Aini Hasmani, Mohamed Hadzri
author_facet	Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hamzah, Hairul Aini Hasmani, Mohamed Hadzri
author_sort	Mustafa Mahmoud, Mohammed Imad Al-Deen
title	Biodynamics of HCV infection in haemodialysis patients in Pahang
title_short	Biodynamics of HCV infection in haemodialysis patients in Pahang
title_full	Biodynamics of HCV infection in haemodialysis patients in Pahang
title_fullStr	Biodynamics of HCV infection in haemodialysis patients in Pahang
title_full_unstemmed	Biodynamics of HCV infection in haemodialysis patients in Pahang
title_sort	biodynamics of hcv infection in haemodialysis patients in pahang
publishDate	2011
url	http://irep.iium.edu.my/11347/ http://irep.iium.edu.my/11347/ http://irep.iium.edu.my/11347/1/IRIE_2011_HCV_Biodynamics.pdf
first_indexed	2023-09-18T20:20:41Z
last_indexed	2023-09-18T20:20:41Z
_version_	1777408086225453056

Biodynamics of HCV infection in haemodialysis patients in Pahang

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