Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study

Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study

Dilated intercellular space (DIS) is considered as early signs of acid damage to the esophageal epithelium. The diffusion of refluxed gastric acid into the intercellular space activates chemo sensitive nociceptors whose signals are transmitted via the spinal cord to the brain for symptom (heartburn)...

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Main Authors: Myint, Yi Yi, Tun, Ye, Mohammad Amjad, Nasser, Mat Hassan, Mohd. Zailani
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
QM Human anatomy
Online Access:http://irep.iium.edu.my/10885/
http://irep.iium.edu.my/10885/
http://irep.iium.edu.my/10885/1/oral_presentation.pdf
id iium-10885
recordtype eprints
spelling iium-108852012-03-28T04:45:22Z http://irep.iium.edu.my/10885/ Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study Myint, Yi Yi Tun, Ye Mohammad Amjad, Nasser Mat Hassan, Mohd. Zailani QM Human anatomy Dilated intercellular space (DIS) is considered as early signs of acid damage to the esophageal epithelium. The diffusion of refluxed gastric acid into the intercellular space activates chemo sensitive nociceptors whose signals are transmitted via the spinal cord to the brain for symptom (heartburn) perception as well as initiated a short reflex arc to esophageal (longitudinal) smooth muscle as means of precipitating a sustained esophageal contraction. Objectives: This study aims to evaluate the intercellular space of oesophageal epithelium and to find out the association between clinical symptoms and DIS among Malaysian with NERD. Materials and methods: The study group included 8 NERD patients with reflux symptoms, normal mucosa on endoscopy and positive 24-hour pH monitoring and 5 normal controls. Specimens were routinely processed for transmission electron microscopy (TEM). The intercellular spaces between prickle cells were viewed and their widths were determined by TEM. Statistical analysis: Data were analyzed using the Statistical Package for Social Science (SPSS) version 16. The difference in mean DIS among NERD patients and controls was tested using independent t test. The association between categorical data and dilatation of intercellular space was tested using Chi-Square test. P-value of less than 0.05 was taken as significant. Results: Morphometric analysis performed on transmission electron microscopy microphotographs showed mean intercellular space diameter values of NERD patients were significantly ( two times) higher than that in normal controls (p<0.001). Intercellular space with width above > 0.93 μm is considered as dilated (cut-off value). There was strong association between dilated intercellular space with heartburn (p 0.001) and not with acid regurgitation (p 0.075). Conclusions: We can conclude that DIS at prickle cell layer is a promising diagnostic finding of early damage of esophageal epithelium. 2010-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/10885/1/oral_presentation.pdf Myint, Yi Yi and Tun, Ye and Mohammad Amjad, Nasser and Mat Hassan, Mohd. Zailani (2010) Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study. In: 19th Scientific Conference of the Electron Microscopy Society of Malaysia & 20th Annual Meeting of Electron Microscopy Society Malaysia (EMSM), 14-16 Dec 2010, Langkawi, Kedah. (Unpublished) http://www.sirim.my/emsm2010/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QM Human anatomy
spellingShingle QM Human anatomy
Myint, Yi Yi
Tun, Ye
Mohammad Amjad, Nasser
Mat Hassan, Mohd. Zailani
Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study
description Dilated intercellular space (DIS) is considered as early signs of acid damage to the esophageal epithelium. The diffusion of refluxed gastric acid into the intercellular space activates chemo sensitive nociceptors whose signals are transmitted via the spinal cord to the brain for symptom (heartburn) perception as well as initiated a short reflex arc to esophageal (longitudinal) smooth muscle as means of precipitating a sustained esophageal contraction. Objectives: This study aims to evaluate the intercellular space of oesophageal epithelium and to find out the association between clinical symptoms and DIS among Malaysian with NERD. Materials and methods: The study group included 8 NERD patients with reflux symptoms, normal mucosa on endoscopy and positive 24-hour pH monitoring and 5 normal controls. Specimens were routinely processed for transmission electron microscopy (TEM). The intercellular spaces between prickle cells were viewed and their widths were determined by TEM. Statistical analysis: Data were analyzed using the Statistical Package for Social Science (SPSS) version 16. The difference in mean DIS among NERD patients and controls was tested using independent t test. The association between categorical data and dilatation of intercellular space was tested using Chi-Square test. P-value of less than 0.05 was taken as significant. Results: Morphometric analysis performed on transmission electron microscopy microphotographs showed mean intercellular space diameter values of NERD patients were significantly ( two times) higher than that in normal controls (p<0.001). Intercellular space with width above > 0.93 μm is considered as dilated (cut-off value). There was strong association between dilated intercellular space with heartburn (p 0.001) and not with acid regurgitation (p 0.075). Conclusions: We can conclude that DIS at prickle cell layer is a promising diagnostic finding of early damage of esophageal epithelium.
format Conference or Workshop Item
author Myint, Yi Yi
Tun, Ye
Mohammad Amjad, Nasser
Mat Hassan, Mohd. Zailani
author_facet Myint, Yi Yi
Tun, Ye
Mohammad Amjad, Nasser
Mat Hassan, Mohd. Zailani
author_sort Myint, Yi Yi
title Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study
title_short Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study
title_full Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study
title_fullStr Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study
title_full_unstemmed Dilated Intercellular Spaces of Esophageal Epithelium among Malaysian With Nonerosive Reflux Disease: Transmission Electron Microscopic (Tem) study
title_sort dilated intercellular spaces of esophageal epithelium among malaysian with nonerosive reflux disease: transmission electron microscopic (tem) study
publishDate 2010
url http://irep.iium.edu.my/10885/
http://irep.iium.edu.my/10885/
http://irep.iium.edu.my/10885/1/oral_presentation.pdf
first_indexed 2023-09-18T20:20:14Z
last_indexed 2023-09-18T20:20:14Z
_version_ 1777408058315505664

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