An isolated bacterial consortium for crude oil biodegradation

Crude oil utilizing bacteria was isolated from crude-oil contaminated soil (COCS) and tested for their ability to degrade COCS. Fifty strains were isolated, purified and monitored on agar plated for their growth. Twenty strains selected for the second monitoring in shake flask using minimal salt med...

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Main Authors: Al-Khatib, Ma An Fahmi Rashid, Alam, Md. Zahangir, Muyibi, Suleyman Aremu, Husain, Iman A. F.
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
Online Access:http://irep.iium.edu.my/10852/
http://irep.iium.edu.my/10852/
http://irep.iium.edu.my/10852/4/an_isolated_bacterial_consortium_for_crude_oil_biodegradation.pdf
id iium-10852
recordtype eprints
spelling iium-108522013-07-03T02:18:28Z http://irep.iium.edu.my/10852/ An isolated bacterial consortium for crude oil biodegradation Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Husain, Iman A. F. TP248.13 Biotechnology Crude oil utilizing bacteria was isolated from crude-oil contaminated soil (COCS) and tested for their ability to degrade COCS. Fifty strains were isolated, purified and monitored on agar plated for their growth. Twenty strains selected for the second monitoring in shake flask using minimal salt medium with crude oil as sole carbon source. The growth was monitored by measuring the optical density (OD600) on daily basis. The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined with accordance to their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. The first order kinetic study showed that the μmax of Cons.1 was 0.02 hr-1 while the half saturation constant ks was 2.2 %v/v TPH. The consortium proved a good biodegradation of crude oil in soil slurry with removal up to 95% which would further be used for the bioremediation of COCS. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/10852/4/an_isolated_bacterial_consortium_for_crude_oil_biodegradation.pdf Al-Khatib, Ma An Fahmi Rashid and Alam, Md. Zahangir and Muyibi, Suleyman Aremu and Husain, Iman A. F. (2011) An isolated bacterial consortium for crude oil biodegradation. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Al-Khatib, Ma An Fahmi Rashid
Alam, Md. Zahangir
Muyibi, Suleyman Aremu
Husain, Iman A. F.
An isolated bacterial consortium for crude oil biodegradation
description Crude oil utilizing bacteria was isolated from crude-oil contaminated soil (COCS) and tested for their ability to degrade COCS. Fifty strains were isolated, purified and monitored on agar plated for their growth. Twenty strains selected for the second monitoring in shake flask using minimal salt medium with crude oil as sole carbon source. The growth was monitored by measuring the optical density (OD600) on daily basis. The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined with accordance to their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. The first order kinetic study showed that the μmax of Cons.1 was 0.02 hr-1 while the half saturation constant ks was 2.2 %v/v TPH. The consortium proved a good biodegradation of crude oil in soil slurry with removal up to 95% which would further be used for the bioremediation of COCS.
format Conference or Workshop Item
author Al-Khatib, Ma An Fahmi Rashid
Alam, Md. Zahangir
Muyibi, Suleyman Aremu
Husain, Iman A. F.
author_facet Al-Khatib, Ma An Fahmi Rashid
Alam, Md. Zahangir
Muyibi, Suleyman Aremu
Husain, Iman A. F.
author_sort Al-Khatib, Ma An Fahmi Rashid
title An isolated bacterial consortium for crude oil biodegradation
title_short An isolated bacterial consortium for crude oil biodegradation
title_full An isolated bacterial consortium for crude oil biodegradation
title_fullStr An isolated bacterial consortium for crude oil biodegradation
title_full_unstemmed An isolated bacterial consortium for crude oil biodegradation
title_sort isolated bacterial consortium for crude oil biodegradation
publishDate 2011
url http://irep.iium.edu.my/10852/
http://irep.iium.edu.my/10852/
http://irep.iium.edu.my/10852/4/an_isolated_bacterial_consortium_for_crude_oil_biodegradation.pdf
first_indexed 2023-09-18T20:20:13Z
last_indexed 2023-09-18T20:20:13Z
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