Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry

Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factor...

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Main Authors:	Mahdi, Harith Jameel, Budi Muljono, Retno Andayani, -, Ishak
Format:	Article
Language:	English
Published:	Science Publications 2010
Subjects:	Q Science (General) RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/10627/ http://irep.iium.edu.my/10627/1/PUB2.PDF

id	iium-10627
recordtype	eprints
spelling	iium-106272012-01-13T09:21:20Z http://irep.iium.edu.my/10627/ Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry Mahdi, Harith Jameel Budi Muljono, Retno Andayani -, Ishak Q Science (General) RS Pharmacy and materia medica Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis. Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivars. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites. Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated. Science Publications 2010 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/10627/1/PUB2.PDF Mahdi, Harith Jameel and Budi Muljono, Retno Andayani and -, Ishak (2010) Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry. American Journal of Applied Science, 7 (1). pp. 17-23. ISSN 1546-9239
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) RS Pharmacy and materia medica
spellingShingle	Q Science (General) RS Pharmacy and materia medica Mahdi, Harith Jameel Budi Muljono, Retno Andayani -, Ishak Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
description	Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis. Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivars. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites. Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated.
format	Article
author	Mahdi, Harith Jameel Budi Muljono, Retno Andayani -, Ishak
author_facet	Mahdi, Harith Jameel Budi Muljono, Retno Andayani -, Ishak
author_sort	Mahdi, Harith Jameel
title	Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_short	Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_full	Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_fullStr	Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_full_unstemmed	Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
title_sort	metabolic fingerprinting of three malaysian ginger (zingiber officinale roscoe) using gas chromatography-mass spectromertry
publisher	Science Publications
publishDate	2010
url	http://irep.iium.edu.my/10627/ http://irep.iium.edu.my/10627/1/PUB2.PDF
first_indexed	2023-09-18T20:20:01Z
last_indexed	2023-09-18T20:20:01Z
_version_	1777408044568674304

Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry

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