Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry
Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factor...
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| Format: | Article |
| Language: | English |
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Science Publications
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/10627/ http://irep.iium.edu.my/10627/1/PUB2.PDF |
| Summary: | Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factors.
Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis.
Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivars. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites.
Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated.
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