An in vivo implantation study in New Zealand white rabbits for granular hydroxyapatite
We developed hydroxyapatite (HA) granules made from local raw materials (GranuMaS™), which have been fabricated using a novel method (Patent (Pending) No. PI 2004 0748). GranuMaS™ have been fully characterized using X-ray diffraction, scanning electron microscopy, energy dispersive x-ray, Four...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AO Research Institute Davos
2008
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/10382/ http://irep.iium.edu.my/10382/ http://irep.iium.edu.my/10382/1/v016supp02a055.pdf |
| Summary: | We developed hydroxyapatite (HA)
granules made from local raw materials (GranuMaS™),
which have been fabricated using a novel method
(Patent (Pending) No. PI 2004 0748). GranuMaS™
have been fully characterized using X-ray diffraction,
scanning electron microscopy, energy dispersive x-ray,
Fourier transform infra-red spectroscopy and
inductively coupled plasma methods. It has also been
shown to comply with the ASTM F1185 - 88 (1993)
specification standards.
Biocompatibility studies have been performed on the
product, which include neutral red cytotoxicity assay
using cultured fibroblast and CRL-1427 osteoblast cells;
MTT (Tetrazolium Salt) assay; cytotoxicity studies for
apoptosis using Acridine Orange / Propidium Iodide
(AO/PI) dual staining of V79 cells in DMEM; and
COMET assay genotoxicity studies using L-929
(normal mouse epithelial) cells.
An in vivo implantation study involving New Zealand
White rabbits were carried out in the course of this
study. |
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