Natural quinolone antimicrobial agents from Malaysian Rutaceous species

The overwhelming majority of naturally occurring quinoline alkaloids are anthranilate-derived alkaloids. Various quinoline alkaloids with diverse structural types have been isolated and most of these alkaloids are typical and unique to the Rutaceae plant family. Some of these alkaloids especially wh...

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Main Authors:	Mohd Hassan, Norazian, Zakaria, Muhamad, Md Taib, Nurhaya, Mohd Kamal, Laina Zarisa, Abdul Karim, Khairul Anuar
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	QD Chemistry QR180 Immunology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/10026/ http://irep.iium.edu.my/10026/ http://irep.iium.edu.my/10026/1/LECTURE.pdf

id	iium-10026
recordtype	eprints
spelling	iium-100262012-02-23T07:38:07Z http://irep.iium.edu.my/10026/ Natural quinolone antimicrobial agents from Malaysian Rutaceous species Mohd Hassan, Norazian Zakaria, Muhamad Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Abdul Karim, Khairul Anuar QD Chemistry QR180 Immunology RS Pharmacy and materia medica The overwhelming majority of naturally occurring quinoline alkaloids are anthranilate-derived alkaloids. Various quinoline alkaloids with diverse structural types have been isolated and most of these alkaloids are typical and unique to the Rutaceae plant family. Some of these alkaloids especially which possess 4-oxygenated and 2-substituted 4-quinolone structures have been demonstrated to have promising antimicrobial activity. Three Malaysian Rutaceous species have been investigated for their natural quinolone antimicrobial agents, namely Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco. Bioassay-guided isolation facilitated by using bioautographic agar overlay assay against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35213 and Candida albicans ATCC 90028 as the indicator microbes has led to the isolation of antimicrobial 4-quinozolone, identified as arborine, a 4-quinolone with the trivial name graveoline and a 2-quinolone namely lunidonine from G. pentaphylla, R. angustifolia and L. amara, respectively. These alkaloids possessed promising antimicrobial activity with the minimum inhibitory concentrations (MICs) ranged between 125 µg/ml and 250 µg/ml against the tested microbes. Arborine and graveoline showed synergistic antimicrobial effect when tested in combination with ampicillin against E. coli with FIC index of 0.38 for each combination. Because of some structural similarity of these alkaloids with that of 4-quinolone antimicrobial agents, the alkaloids are suggested to be the natural quinolone antimicrobial agents which have the same cellular target as the synthetic agents, which is the bacterial DNA. The study on DNA binding ability of these alkaloids is ongoing. 2010-10-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/10026/1/LECTURE.pdf Mohd Hassan, Norazian and Zakaria, Muhamad and Md Taib, Nurhaya and Mohd Kamal, Laina Zarisa and Abdul Karim, Khairul Anuar (2010) Natural quinolone antimicrobial agents from Malaysian Rutaceous species. In: 3rd International Conference on Biotechnology for the Wellness Industry & Exhibition, 8-9 October 2010, Kuala Lumpur, Malaysia. http://www.cepp.utm.my/icbwi10
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry QR180 Immunology RS Pharmacy and materia medica
spellingShingle	QD Chemistry QR180 Immunology RS Pharmacy and materia medica Mohd Hassan, Norazian Zakaria, Muhamad Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Abdul Karim, Khairul Anuar Natural quinolone antimicrobial agents from Malaysian Rutaceous species
description	The overwhelming majority of naturally occurring quinoline alkaloids are anthranilate-derived alkaloids. Various quinoline alkaloids with diverse structural types have been isolated and most of these alkaloids are typical and unique to the Rutaceae plant family. Some of these alkaloids especially which possess 4-oxygenated and 2-substituted 4-quinolone structures have been demonstrated to have promising antimicrobial activity. Three Malaysian Rutaceous species have been investigated for their natural quinolone antimicrobial agents, namely Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco. Bioassay-guided isolation facilitated by using bioautographic agar overlay assay against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35213 and Candida albicans ATCC 90028 as the indicator microbes has led to the isolation of antimicrobial 4-quinozolone, identified as arborine, a 4-quinolone with the trivial name graveoline and a 2-quinolone namely lunidonine from G. pentaphylla, R. angustifolia and L. amara, respectively. These alkaloids possessed promising antimicrobial activity with the minimum inhibitory concentrations (MICs) ranged between 125 µg/ml and 250 µg/ml against the tested microbes. Arborine and graveoline showed synergistic antimicrobial effect when tested in combination with ampicillin against E. coli with FIC index of 0.38 for each combination. Because of some structural similarity of these alkaloids with that of 4-quinolone antimicrobial agents, the alkaloids are suggested to be the natural quinolone antimicrobial agents which have the same cellular target as the synthetic agents, which is the bacterial DNA. The study on DNA binding ability of these alkaloids is ongoing.
format	Conference or Workshop Item
author	Mohd Hassan, Norazian Zakaria, Muhamad Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Abdul Karim, Khairul Anuar
author_facet	Mohd Hassan, Norazian Zakaria, Muhamad Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Abdul Karim, Khairul Anuar
author_sort	Mohd Hassan, Norazian
title	Natural quinolone antimicrobial agents from Malaysian Rutaceous species
title_short	Natural quinolone antimicrobial agents from Malaysian Rutaceous species
title_full	Natural quinolone antimicrobial agents from Malaysian Rutaceous species
title_fullStr	Natural quinolone antimicrobial agents from Malaysian Rutaceous species
title_full_unstemmed	Natural quinolone antimicrobial agents from Malaysian Rutaceous species
title_sort	natural quinolone antimicrobial agents from malaysian rutaceous species
publishDate	2010
url	http://irep.iium.edu.my/10026/ http://irep.iium.edu.my/10026/ http://irep.iium.edu.my/10026/1/LECTURE.pdf
first_indexed	2023-09-18T20:19:32Z
last_indexed	2023-09-18T20:19:32Z
_version_	1777408013762560000

Natural quinolone antimicrobial agents from Malaysian Rutaceous species

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